PMID- 10321491
OWN - NLM
STAT- MEDLINE
DCOM- 19990623
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 10
IP  - 5
DP  - 1999 Apr 6
TI  - NMDA induces BDNF expression in the albino rat retina in vivo.
PG  - 1103-6
AB  - The effect of an intravitreal injection of NMDA on the expression of 
      brain-derived neurotrophic factor (BDNF) in retinal ganglion cells was 
      investigated in rats. Forty-eight hours after intravitreal injection of NMDA 
      retinal ganglion cell BDNF immunoreactivity was practically obliterated, as was 
      the choline acetyltransferase (ChAT) immunoreactivity associated with a subset of 
      amacrine cells. However, 2h following treatment with NMDA the BDNF 
      immunoreactivity and BDNF mRNA associated with the ganglion cells was enhanced 
      while the amacrine cell ChAT immunoreactivity was clearly reduced and the levels 
      of mRNA coding for rhodopsin and Thy-1 did not change. However, 4h after NMDA 
      injection the increase in BDNF mRNA was now no longer apparent. The results show 
      that synthesis of BDNF is increased in the ganglion cells immediately following 
      an insult by NMDA. It is suggested that this is a natural protective mechanism of 
      rat retinal ganglion cells.
FAU - Vecino, E
AU  - Vecino E
AD  - Departamento de Biologia Celular y Ciencias Morfologicas, Facultad de Medicina, 
      Universidad del Pais Vasco, Leioa, Vizcaya, Spain.
FAU - Ugarte, M
AU  - Ugarte M
FAU - Nash, M S
AU  - Nash MS
FAU - Osborne, N N
AU  - Osborne NN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Choline O-Acetyltransferase/metabolism
MH  - Immunohistochemistry
MH  - N-Methylaspartate/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Retina/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1097/00001756-199904060-00036 [doi]
PST - ppublish
SO  - Neuroreport. 1999 Apr 6;10(5):1103-6. doi: 10.1097/00001756-199904060-00036.