PMID- 10325260
OWN - NLM
STAT- MEDLINE
DCOM- 19990714
LR  - 20190513
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 14
IP  - 5
DP  - 1999 May
TI  - Elevated expression of activation molecules by decidual lymphocytes in women 
      suffering spontaneous early pregnancy loss.
PG  - 1194-200
AB  - The purpose of this study was to investigate, quantify and compare the expression 
      of activation markers by decidual leukocytes in sporadic spontaneous early 
      pregnancy loss and apparently normal first trimester human pregnancy. Decidua was 
      obtained from 18 therapeutic abortions and 20 sporadic spontaneous abortions at 
      8-12 weeks gestational age. Cryostat sections were labelled by the avidin-biotin 
      complex-peroxidase method using monoclonal antibodies specific for CD45, CD56, 
      CD3, human leukocyte antigen (HLA) DR, CD69, CD25 and very late antigen (VLA)1. 
      Positive cells were quantified and the results were analysed using the 
      Mann-Whitney statistical test. Significantly increased numbers of CD69-positive 
      and CD25-positive cells were detected in spontaneous abortion decidua, when 
      compared with therapeutic abortion decidua. Approximately 50% of women 
      experiencing spontaneous miscarriage also contained significantly elevated 
      numbers of HLA DR-positive cells within decidua. Double immunohistochemical 
      labelling studies demonstrated that the CD25-positive and CD69-positive cells in 
      spontaneous abortion decidua were CD3-positive T cells rather than CD56-positive 
      granulated lymphocytes. Immunological dysfunction within endometrium may account 
      for a proportion of sporadic spontaneous abortions.
FAU - Vassiliadou, N
AU  - Vassiliadou N
AD  - Department of Pathology, University of Newcastle, Royal Victoria Infirmary, 
      Newcastle upon TyneNE1 4LP, UK.
FAU - Searle, R F
AU  - Searle RF
FAU - Bulmer, J N
AU  - Bulmer JN
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Biomarkers)
RN  - 0 (CD69 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Abortion, Spontaneous/*blood
MH  - Antigens, CD/blood
MH  - Antigens, Differentiation, T-Lymphocyte/blood
MH  - Biomarkers/blood
MH  - Decidua/*immunology
MH  - Female
MH  - HLA-DR Antigens/blood
MH  - Humans
MH  - Immunohistochemistry
MH  - Lectins, C-Type
MH  - *Lymphocyte Activation
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Receptors, Interleukin-2/blood
EDAT- 1999/05/15 00:00
MHDA- 1999/05/15 00:01
CRDT- 1999/05/15 00:00
PHST- 1999/05/15 00:00 [pubmed]
PHST- 1999/05/15 00:01 [medline]
PHST- 1999/05/15 00:00 [entrez]
AID - 10.1093/humrep/14.5.1194 [doi]
PST - ppublish
SO  - Hum Reprod. 1999 May;14(5):1194-200. doi: 10.1093/humrep/14.5.1194.