PMID- 10325509
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20180213
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 35
IP  - 5-6
DP  - 1999
TI  - Genetic and chromosomal alterations in prostatic intraepithelial neoplasia and 
      carcinoma detected by fluorescence in situ hybridization.
PG  - 479-83
AB  - OBJECTIVE: In this review, we discuss the utility of fluorescence in situ 
      hybridization (FISH) in the determination of genetic and chromosomal alterations 
      in prostate cancer specimens. We also discuss the genetic association between 
      prostatic intraepithelial neoplasia (PIN) and adenocarcinoma as detected by FISH 
      and other techniques. METHODS AND RESULTS: FISH is a commonly used technique for 
      the determination of gene and chromosome dosage. In tissue sections, FISH allows 
      precise histopathologic correlation of multiple foci of normal epithelium, 
      premalignant lesions, and carcinoma within a single specimen, including study of 
      intratumoral heterogeneity. PIN and prostatic carcinoma foci have a similar 
      proportion of genetic changes, but foci of carcinoma usually have more 
      alterations. This supports the hypothesis that PIN is the most likely precursor 
      of prostatic carcinoma. The most common genetic alterations in PIN and carcinoma 
      are: (1) gain of chromosome 7, particularly 7q31; (2) loss of 8p and gain of 8q, 
      and (3) loss of 10q, 16q and 18q. Inactivation of tumor suppressor genes and/or 
      overexpression of oncogenes in these regions may be important for the initiation 
      and progression of prostate cancer. CONCLUSIONS: FISH is a useful technique to 
      determine genetic relationships between cancer and its precursors. PIN and 
      prostatic carcinoma foci have a similar proportion of genetic alterations, 
      suggesting that PIN is often a precursor of prostatic carcinoma. Genes on 
      chromosomes 7, 8, 10, 16 and 18 may play an important role in both initiation and 
      progression of prostatic carcinoma.
FAU - Qian, J
AU  - Qian J
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minn. 
      55905, USA.
FAU - Jenkins, R B
AU  - Jenkins RB
FAU - Bostwick, D G
AU  - Bostwick DG
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Carcinoma in Situ/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Culture Techniques
MH  - Diagnosis, Differential
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Sensitivity and Specificity
RF  - 35
EDAT- 1999/05/15 02:01
MHDA- 2000/08/16 11:00
CRDT- 1999/05/15 02:01
PHST- 1999/05/15 02:01 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1999/05/15 02:01 [entrez]
AID - 19883 [pii]
AID - 10.1159/000019883 [doi]
PST - ppublish
SO  - Eur Urol. 1999;35(5-6):479-83. doi: 10.1159/000019883.