PMID- 10326586
OWN - NLM
STAT- MEDLINE
DCOM- 19990526
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 111
IP  - 1
DP  - 1999 May
TI  - Investigation of chromosomal aberrations in hepatocellular carcinoma by 
      fluorescence in situ hybridization.
PG  - 21-7
AB  - Molecular cytogenetic approaches have been applied only rarely in the 
      characterization of hepatocellular carcinoma (HCC). The aim in this study was to 
      evaluate aberrations, particularly deletions, of specific chromosomal regions in 
      HCC. Dual-color fluorescence in situ hybridization (FISH) was performed on intact 
      nuclei from touch preparations of 17 HCCs and 1 hepatic adenoma. Each touch 
      preparation was hybridized with a digoxigenin-labeled centromere probe and a 
      biotin-labeled unique sequence probe from the same chromosome. This approach 
      permitted the simultaneous evaluation of ploidy changes and chromosome arm 
      deletions. Eight noncentromeric chromosome regions, 3p14, 4q21, 6q14, 6q21, 8p12, 
      8p22, 9p21, and 9p24 were selected for study on the basis of their having been 
      implicated as tumor suppressor regions in HCC or other common types of carcinoma. 
      Together with the 5 corresponding centromeric probes on chromosomes 3, 4, 6, 8, 
      and 9, a total of 13 chromosome loci were evaluated. All cases of hepatocellular 
      carcinoma showed at least one deletion or aneuploidy. The hepatic adenoma was all 
      diploid. Chromosome 4q21 showed the highest rate of deletion (76.5%) and aneusomy 
      (88%). The second and the third were chromosome 8p22 and 6q14, which showed 59% 
      and 47% of deletion, respectively. A 4q21 deletion is also the most frequent 
      single chromosome aberration. Prominent tumor heterogeneity and variable deletion 
      patterns were noted. Interphase FISH was an efficient means for evaluating 
      numerical and structural chromosome aberrations in HCCs. Most HCCs contained 
      deletions of known tumor suppressor regions (4q and 8p), and a novel deletion 
      hotspot was demonstrated on chromosome band 6q14.
FAU - Huang, S F
AU  - Huang SF
AD  - Department of Pathology, National Taiwan University Hospital, National Taiwan 
      University College of Medicine, Taipei, Republic of China.
FAU - Hsu, H C
AU  - Hsu HC
FAU - Fletcher, J A
AU  - Fletcher JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Female
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0165460898002155 [pii]
AID - 10.1016/s0165-4608(98)00215-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 May;111(1):21-7. doi: 10.1016/s0165-4608(98)00215-5.