PMID- 10327166
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 265
IP  - 3
DP  - 1999 Apr 23
TI  - Enhanced expression of neurotrophic factors by C6 rat glioma cells after 
      transfection with glia maturation factor.
PG  - 203-6
AB  - Glia maturation factor (GMF) is a 17-kDa protein unique to the nervous system. 
      Although GMF was initially characterized as a growth/differentiation factor, the 
      absence of a leader sequence and its intracellular localization in normal brain 
      suggest an intracellularfunction as well. In this paper we transfected the C6 
      glioma cells with GMF cDNA by infecting the cells with a GMF/adenovirus 
      construct. The transfected cells overexpressed GMF but did not secret the protein 
      into the culture medium. However, the transfected cells showed an increased 
      expression of the neurotrophic factors including nerve growth factor (NGF) and 
      brain-derived neurotrophic factor (BDNF). The increase in neurotrophic activity 
      of the C6 cell conditioned medium was demonstrable by its ability to promote 
      neurite outgrowth in PC12 cells.
FAU - Zaheer, A
AU  - Zaheer A
AD  - Department of Neurology, University of Iowa College of Medicine and Veterans 
      Affairs Medical Center, Iowa City 52242, USA.
FAU - Weiss, J L
AU  - Weiss JL
FAU - Goyal, P
AU  - Goyal P
FAU - Lim, R
AU  - Lim R
LA  - eng
GR  - DK-25295/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Glia Maturation Factor)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Culture Media, Conditioned/pharmacology
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Expression Regulation, Viral
MH  - Glia Maturation Factor
MH  - *Glioma
MH  - Growth Inhibitors/*genetics
MH  - Lac Operon
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurites/physiology
MH  - Neurons/cytology/ultrastructure
MH  - PC12 Cells
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - Tumor Cells, Cultured/drug effects/physiology
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0304394099002530 [pii]
AID - 10.1016/s0304-3940(99)00253-0 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Apr 23;265(3):203-6. doi: 10.1016/s0304-3940(99)00253-0.