PMID- 10327576
OWN - NLM
STAT- MEDLINE
DCOM- 19990621
LR  - 20071115
IS  - 0044-409X (Print)
IS  - 0044-409X (Linking)
VI  - 124
IP  - 3
DP  - 1999
TI  - [Production of chemokines by the human peritoneum].
PG  - 206-9
AB  - AIM OF THE STUDY: Peritonitis is characterised by a continued infiltration of the 
      peritoneal cavity with leukocytes, attracted by chemotactic mediators. The aim of 
      this study was to demonstrate the capacity of the human peritoneum to secrete 
      chemokines and to show a therapeutic option by impairing the proinflammatory 
      function of the peritoneum. METHODS: Peritoneum was obtained from 12 consenting 
      patients undergoing abdominal surgery for noninflammatory diseases. After opening 
      the peritoneal cavity a piece of the parietal peritoneum was excised and 
      subsequently incubated with lipopolysaccharide (LPS, 50 ng/ml) +/- interleukin-10 
      (IL-10, 100 U/ml) for five hours in vitro. The culture supernatants were assayed 
      for concentrations of interleukin-8 (IL-8), monocyte chemoattractant protein-1 
      (MCP-1) and monocyte inflammatory protein-1 alpha (MIP-1 alpha) by using the 
      ELISA. RESULTS: The cultured peritoneum secreted MCP-1 (mean (SEM): 3416 (659) 
      pg/ml) and IL-8 (2946 (894) pg/ml). The presence of LPS resulted in a fourfold 
      enhancement of this secretion (MCP-1: 13563 (1613), IL-8: 9854 (1305) pg/ml) and 
      led to the production of MIP-1 alpha (1476 (240) pg/ml). The LPS-stimulated 
      production of all of these chemokines was significantly diminished by the 
      presence of IL-10. CONCLUSION: The reaction of the peritoneum to LPS indicates 
      its proinflammatory function in the context of peritonitis caused by 
      gram-negative bacteria. This inflammatory reaction might be diminished by 
      application of IL-10.
FAU - Riese, J
AU  - Riese J
AD  - Chirurgische Klinik mit Poliklinik, Universitat Erlangen-Nurnberg.
FAU - Denzel, C
AU  - Denzel C
FAU - Zowe, M
AU  - Zowe M
FAU - Mehler, C
AU  - Mehler C
FAU - Hohenberger, W
AU  - Hohenberger W
FAU - Haupt, W
AU  - Haupt W
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Das humane Peritoneum als Produzent von Chemokinen.
PL  - Germany
TA  - Zentralbl Chir
JT  - Zentralblatt fur Chirurgie
JID - 0413645
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokines)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Chemokine CCL2/analysis
MH  - Chemokine CCL4
MH  - Chemokines/*biosynthesis
MH  - Culture Media, Conditioned/chemistry
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-10/pharmacology
MH  - Interleukin-8/analysis
MH  - Lipopolysaccharides/antagonists & inhibitors/pharmacology
MH  - Macrophage Inflammatory Proteins/analysis
MH  - Peritoneum/cytology/*metabolism
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
PST - ppublish
SO  - Zentralbl Chir. 1999;124(3):206-9.