PMID- 10328854
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20071115
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 22
IP  - 6
DP  - 1998
TI  - Regulation of retinoid X receptor-gamma gene transcript levels in rat heart 
      cells.
PG  - 457-63
AB  - Retinoid X receptor-gamma (RXRgamma) is a transcription factor that mediates 
      retinoid signalling and is expressed in rat heart during adult life. However, its 
      expression in embryonic and neonatal heart has not been investigated and it is 
      not known whether ventricular cardiomyocytes express RXRgamma or whether all- 
      trans -retinoic acid (tRA) and thyroid hormone (T3) could influence RXRgamma 
      transcript levels in these cells. First, in situ hybridization experiments were 
      used to test for any spatio-temporal correlation between RXRgamma gene expression 
      and the previously shown requirement for retinoid signalling in embryonic 
      ventricular cardiomyocytes. It was shown that RXRgamma transcripts are not 
      detectable in embryonic heart at all developmental stages examined under 
      conditions where they are detectable in other embryonic tissues. Second, Northern 
      blotting was used to examine whether there is a difference in RXRgamma transcript 
      levels between neonatal and adult heart. We show that levels of two RXRgamma 
      transcripts are developmentally regulated during the postnatal period because 
      they differ between neonatal and adult hearts. Third, it was demonstrated that 
      primary cultures of neonatal rat ventricular cardiomyocytes express RXRgamma 
      transcripts, making them a novel in vitro system for the study of RXRgamma gene 
      regulation in heart-derived cells. Finally, this system was used to examine 
      whether tRA and T3can influence levels of RXRgamma transcripts because they have 
      been shown to have antagonistic effects in this system and to influence RXRgamma 
      RNA levels in other systems. It was shown by Northern blot experiments, that in 
      this system, RXRgamma transcript levels are differentially influenced by these 
      two hormones. The significance of these findings in relation to previously 
      published work is discussed.
CI  - Copyright 1998 Academic Press.
FAU - Georgiades, P
AU  - Georgiades P
AD  - Medical Molecular Biology Unit, Department of Molecular Pathology, University 
      College London Medical School, 46 Cleveland Street, London, W1P 6DB, U.K.
FAU - Brickell, P M
AU  - Brickell PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Animals, Newborn
MH  - DNA-Binding Proteins/genetics
MH  - Embryo, Mammalian
MH  - Embryonic and Fetal Development
MH  - Fetal Heart/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Heart/growth & development
MH  - Heart Ventricles
MH  - Myocardium/*metabolism
MH  - Rats
MH  - Receptors, Retinoic Acid/*genetics
MH  - Retinoid X Receptors
MH  - Transcription Factors/*genetics
MH  - *Transcription, Genetic
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S1065-6995(98)90282-7 [pii]
AID - 10.1006/cbir.1998.0282 [doi]
PST - ppublish
SO  - Cell Biol Int. 1998;22(6):457-63. doi: 10.1006/cbir.1998.0282.