PMID- 10328976
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20151119
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 68
IP  - 5
DP  - 1999 May
TI  - Rabbit retinal Muller cells undergo antigenic changes in response to 
      experimentally induced proliferative vitreoretinopathy.
PG  - 617-27
AB  - Experimental proliferative vitreoretinopathy (PVR) was induced in the rabbit eye 
      by injecting mitotically active Muller cells into the vitreal chamber. Two weeks 
      after the initiation of PVR, the retina and the epiretinal membrane that formed 
      were examined to ascertain the antigenic expression of Muller cells in the retina 
      and in the epiretinal membrane. Examination of various regions of the retina from 
      the experimental PVR eye demonstrated that vimentin, glial fibrillary acidic 
      protein (GFAP), cellular retinaldehyde binding protein (CRALBP), and beta-amyloid 
      precursor protein (beta-APP), which were present in the Muller cells of the 
      retina from the control eye, increased their expression, while the antigenicity 
      of glutamine synthetase (GS), did not change; these proteins were also present in 
      the cells contained within the experimentally induced epiretinal membrane. Alpha 
      smooth muscle actin (alpha-SMA), a cytoskeletal protein that is associated with 
      migration and tractional forces in many cell types, was not only present in the 
      cells embedded within the epiretinal membrane, but was also present in the Muller 
      cells underlying the epiretinal membrane. However, Muller cells that were in the 
      inferior portion of the retina, where epiretinal membrane pathology was absent, 
      did not express alpha-SMA. Although this protein is not normally found in Muller 
      cells, they do express it de novo when they are maintained in culture. This 
      suggests that a localized mechanism associated with epiretinal membrane formation 
      induces the expression of alpha-SMA in Muller cells while the increased 
      expression of GFAP, beta-APP, vimentin, and CRALBP are probably regulated via a 
      more general mechanism.
CI  - Copyright 1999 Academic Press.
FAU - McGillem, G S
AU  - McGillem GS
AD  - Eye Foundation Hospital, Department of Ophthalmology, University of Alabama at 
      Birmingham, Birmingham, AL, 35233, USA.
FAU - Dacheux, R F
AU  - Dacheux RF
LA  - eng
GR  - EY-03011/EY/NEI NIH HHS/United States
GR  - P30EY03039/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (11-cis-retinal-binding protein)
RN  - 0 (Actins)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Antigens)
RN  - 0 (Biomarkers)
RN  - 0 (Carrier Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Vimentin)
SB  - IM
MH  - Actins/analysis
MH  - Amyloid beta-Protein Precursor/analysis
MH  - Animals
MH  - Antigens/*analysis
MH  - Biomarkers/analysis
MH  - Carrier Proteins/analysis
MH  - Cells, Cultured
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Immunohistochemistry
MH  - Neuroglia/*metabolism/pathology
MH  - Rabbits
MH  - Retina/*metabolism/pathology
MH  - Vimentin/analysis
MH  - Vitreoretinopathy, Proliferative/*metabolism/pathology
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0014-4835(98)90648-0 [pii]
AID - 10.1006/exer.1998.0648 [doi]
PST - ppublish
SO  - Exp Eye Res. 1999 May;68(5):617-27. doi: 10.1006/exer.1998.0648.