PMID- 10329598
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 154
IP  - 5
DP  - 1999 May
TI  - Detection of translocation t(11;14)(q13;q32) in mantle cell lymphoma by 
      fluorescence in situ hybridization.
PG  - 1449-52
AB  - To assess an unequivocal diagnosis of mantle cell lymphoma (MCL), we have 
      developed a fluorescence in situ hybridization (FISH) assay, enabling the 
      demonstration of t(11;14)(q13;q32) directly on pathological samples. We have 
      first selected CCND1 and IGH probes encompassing the breakpoint regions on both 
      chromosomes. Then, we have defined experimental conditions enabling us to obtain 
      bright clear-cut signals in all of the samples, independently of the initial 
      fixation conditions. We have analyzed single-cell suspensions from 26 
      formalin-fixed, paraffin-embedded MCL samples with this set of probes. In all 
      cases, we have found a fusion signal (ie, a t(11;14)(q13;q32) translocation) in 
      14% to 99% of cells (median, 87%). So far, IGH-CCND1 fusions have been detected 
      in all of the 51 MCL patients that we have analyzed by FISH (either on 
      paraffin-embedded tumor samples or on peripheral blood samples). Regarding the 
      low sensitivity of other techniques used to diagnose t(11;14)(q13;q32) (ie, 70% 
      to 75% for cytogenetics and 50% to 60% for polymerase chain reaction), our FISH 
      assay is by far the most sensitive technique. Moreover, because of the quality of 
      the fluorescent signals and the rapidity of the experiment, this technique is 
      widely applicable, even in routine cytogenetics or pathology laboratories. As MCL 
      patients are usually refractory to standard therapy, an unambiguous diagnosis is 
      needed to propose adapted therapeutic strategies, and this highly sensitive assay 
      may be of great value for accurate diagnosis in difficult cases.
FAU - Li, J Y
AU  - Li JY
AD  - Laboratory of Hematology, Laboratory of Pathology, and Clinical Hematology 
      Department, University Hospital, Nantes, France.
FAU - Gaillard, F
AU  - Gaillard F
FAU - Moreau, A
AU  - Moreau A
FAU - Harousseau, J L
AU  - Harousseau JL
FAU - Laboisse, C
AU  - Laboisse C
FAU - Milpied, N
AU  - Milpied N
FAU - Bataille, R
AU  - Bataille R
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Female
MH  - Fixatives
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Non-Hodgkin/*genetics
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - *Translocation, Genetic
PMC - PMC1866594
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
PMCR- 1999/11/01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - S0002-9440(10)65399-0 [pii]
AID - 1699 [pii]
AID - 10.1016/S0002-9440(10)65399-0 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 May;154(5):1449-52. doi: 10.1016/S0002-9440(10)65399-0.