PMID- 10329949
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20190122
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 276
IP  - 5
DP  - 1999 May
TI  - Cloning and expression of the Na+/H+ exchanger from Amphiuma RBCs: resemblance to 
      mammalian NHE1.
PG  - C1025-37
LID - 10.1152/ajpcell.1999.276.5.C1025 [doi]
AB  - The cDNA encoding the Na+/H+ exchanger (NHE) from Amphiuma erythrocytes was 
      cloned, sequenced, and found to be highly homologous to the human NHE1 isoform 
      (hNHE1), with 79% identity and 89% similarity at the amino acid level. Sequence 
      comparisons with other NHEs indicate that the Amphiuma tridactylum NHE isoform 1 
      (atNHE1) is likely to be a phylogenetic progenitor of mammalian NHE1. The atNHE1 
      protein, when stably transfected into the NHE-deficient AP-1 cell line (37), 
      demonstrates robust Na+-dependent proton transport that is sensitive to amiloride 
      but not to the potent NHE1 inhibitor HOE-694. Interestingly, chimeric NHE 
      proteins constructed by exchanging the amino and carboxy termini between atNHE1 
      and hNHE1 exhibited drug sensitivities similar to atNHE1. Based on kinetic, 
      sequence, and functional similarities between atNHE1 and mammalian NHE1, we 
      propose that the Amphiuma exchanger should prove to be a valuable model for 
      studying the control of pH and volume regulation of mammalian NHE1. However, low 
      sensitivity of atNHE1 to the NHE inhibitor HOE-694 in both native Amphiuma red 
      blood cells (RBCs) and in transfected mammalian cells distinguishes this 
      transporter from its mammalian homologue.
FAU - McLean, L A
AU  - McLean LA
AD  - Department of Human Physiology, School of Medicine, University of California, 
      Davis, California 95616, USA.
FAU - Zia, S
AU  - Zia S
FAU - Gorin, F A
AU  - Gorin FA
FAU - Cala, P M
AU  - Cala PM
LA  - eng
SI  - GENBANK/AF144087
GR  - HL-07682/HL/NHLBI NIH HHS/United States
GR  - HL-21179/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (DNA, Complementary)
RN  - 0 (Sodium-Hydrogen Exchangers)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Erythrocytes/*chemistry
MH  - *Gene Expression
MH  - Humans
MH  - Molecular Sequence Data
MH  - Sequence Homology
MH  - Sodium-Hydrogen Exchangers/chemistry/*genetics
MH  - Transfection
MH  - Urodela/*blood
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1152/ajpcell.1999.276.5.C1025 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 May;276(5):C1025-37. doi: 10.1152/ajpcell.1999.276.5.C1025.