PMID- 10330132
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20181113
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 9
IP  - 5
DP  - 1999 May
TI  - Mouse molecular cytogenetic resource: 157 BACs link the chromosomal and genetic 
      maps.
PG  - 514-23
AB  - We have established a collection of strong molecular cytogenetic markers that 
      span the mouse autosomes and X chromosome at an average spacing of one per 19 Mb 
      and identify 127 distinct band landmarks. In addition, this Mouse Molecular 
      Cytogenetic Resource relates the ends of the genetic maps to their chromosomal 
      locations. The resource consists of 157 bacterial artificial chromosome (BAC) 
      clones, each of which identifies specific mouse chromosome bands or band borders, 
      and 42 of which are linked to genetic markers that define the centromeric and 
      telomeric ends of the Whitehead/MIT recombinational maps. In addition, 108 
      randomly selected and 6 STS-linked BACs have been assigned to single chromosome 
      bands. We have also developed a high-resolution fluorescent reverse-banding 
      technique for mouse chromosomes that allows simultaneous localization of probes 
      by fluorescence in situ hybridization (FISH) with respect to the cytogenetic 
      landmarks. This approach integrates studies of the entire mouse genome. Moreover, 
      these reagents will simplify gene mapping and analyses of genomic fragments in 
      fetal and adult mouse models. As shown with the MMU16 telomeric marker for the 
      trisomy 16 mouse model of Down syndrome, these clones can obviate the need for 
      metaphase analyses. The potential contribution of this resource and associated 
      methods extends well beyond mapping and includes clues to understanding mouse 
      chromosomes and their rearrangements in cancers and evolution. Finally it will 
      facilitate the development of an integrated view of the mouse genome by providing 
      anchor points from the genetic to the cytogenetic and functional maps of the 
      mouse as we attempt to understand mutations, their biological consequences, and 
      gene function.
FAU - Korenberg, J R
AU  - Korenberg JR
AD  - Medical Genetics Birth Defects Center, Cedars-Sinai Medical Center, University of 
      California, Los Angeles, Los Angeles, California 90048, USA. 
      julie.korenberg@cshs.org
FAU - Chen, X N
AU  - Chen XN
FAU - Devon, K L
AU  - Devon KL
FAU - Noya, D
AU  - Noya D
FAU - Oster-Granite, M L
AU  - Oster-Granite ML
FAU - Birren, B W
AU  - Birren BW
LA  - eng
GR  - R01 HG00934/HG/NHGRI NIH HHS/United States
GR  - R01 HL50025/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosome Banding/methods
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Bacterial/*genetics
MH  - Genetic Markers/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Telomere/genetics
PMC - PMC310771
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
PMCR- 1999/11/01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
PST - ppublish
SO  - Genome Res. 1999 May;9(5):514-23.