PMID- 10331497
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 65
IP  - 5
DP  - 1999 May
TI  - MCP-1 is selectively expressed in the late phase by cytokine-stimulated human 
      neutrophils: TNF-alpha plays a role in maximal MCP-1 mRNA expression.
PG  - 671-9
AB  - Culture supernatants of phytohemagglutinin (PHA)-stimulated human peripheral 
      blood mononuclear cells (PHA-sup) induced monocyte chemoattractant protein-1 
      (MCP-1) mRNA expression in human neutrophils. MCP-1 mRNA was first detected by 
      Northern analysis at 8 h, and the peak level was detected at 16 h and sustained 
      until 72 h. Cycloheximide and genistein, but not pertussis toxin, inhibited the 
      expression of MCP-1 mRNA. Recombinant tumor necrosis factor alpha (TNF-alpha) 
      induced a low level MCP-1 mRNA accumulation in neutrophils, and addition of 
      anti-TNF-alpha IgG blocked 30-70% of MCP-1 mRNA expression induced with PHA-sup. 
      PHA-sup-stimulated PMN synthesized and secreted 3.1+/-1.3 ng/5 x 10(6) PMN MCP-1 
      within the first 24 h. Hybridization of 32P-labeled cDNA preparations to an array 
      of human cytokine cDNAs further indicated that MCP-1 mRNA was selectively 
      up-regulated in the late phase after stimulation with the PHA-sup.
FAU - Yamashiro, S
AU  - Yamashiro S
AD  - Immunopathology Section, Laboratory of Immunobiology, National Cancer 
      Institute-Frederick Cancer Research and Development Center, Maryland 21702, USA.
FAU - Kamohara, H
AU  - Kamohara H
FAU - Yoshimura, T
AU  - Yoshimura T
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 98600C0908 (Cycloheximide)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Chemokine CCL2/*biosynthesis/*genetics
MH  - Cycloheximide/pharmacology
MH  - Cytokines/*pharmacology
MH  - DNA, Complementary/genetics/metabolism
MH  - Genistein/pharmacology
MH  - Growth Inhibitors/pharmacology
MH  - Humans
MH  - Kinetics
MH  - Neutrophils/drug effects/*metabolism
MH  - Pertussis Toxin
MH  - Phytohemagglutinins/pharmacology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/*biosynthesis/metabolism
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Up-Regulation/immunology
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - 10.1002/jlb.65.5.671 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 May;65(5):671-9. doi: 10.1002/jlb.65.5.671.