PMID- 10331846
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 75
IP  - 4
DP  - 1999 Apr
TI  - Heterogeneity of Chinese hamster X chromosomes in X-ray-induced chromosomal 
      aberrations.
PG  - 419-27
AB  - PURPOSE: To study the frequencies and distribution of X-ray-induced chromosomal 
      aberrations in different arms as well as different regions of the long arms of 
      Chinese hamster X chromosomes by fluorescence in situ hybridization (FISH). 
      MATERIAL AND METHODS: Female embryonic primary cells of Chinese hamster were 
      exposed to 1 and 4 Gy X-rays during the G1 stage and metaphases were collected 20 
      h later with colcemid-blocking. Induced aberrations involving different arms as 
      well as different regions of the long arm of the X chromosomes were analysed by 
      three-colour FISH using arm-specific painting probes. Distributions of 
      aberrations in the arms were statistically analysed by chi2-test, with the 
      hypothesis that aberrations are proportionally distributed on the basis of their 
      relative lengths of the arms. RESULTS: The long arms of the X chromosomes were 
      frequently involved in breaks than was the short arm. The result of the chi2-test 
      indicates a non-proportional distribution of breaks between the arms, while 
      exchanges (dicentrics and translocations) involving the arms were proportionally 
      distributed. Differential involvement of regions of the long arm, i.e. Xq1 and 
      Xq2, in breaks was also observed. Xq21, a known common fragile site in the X 
      chromosome, was often involved in terminal deletions. CONCLUSION: Arm-specific 
      probes of Chinese hamster chromosomes are useful for the detailed study of 
      X-ray-induced aberrations in the X chromosome. The heterogeneity of the Chinese 
      hamster X chromosome in response to X-ray-induced aberrations exists not only 
      between the short (euchromatin) and the long (heterochromatin) arms, but also 
      between different heterochromatic regions of the long arm of the X chromosome.
FAU - Xiao, Y
AU  - Xiao Y
AD  - MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden University 
      Medical Centre, The Netherlands.
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Cricetinae
MH  - Cricetulus
MH  - Dose-Response Relationship, Radiation
MH  - Embryo, Mammalian
MH  - Female
MH  - Heterochromatin/genetics/*radiation effects
MH  - In Situ Hybridization, Fluorescence
MH  - X Chromosome/*radiation effects
MH  - X-Rays
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - 10.1080/095530099140339 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1999 Apr;75(4):419-27. doi: 10.1080/095530099140339.