PMID- 10331967
OWN - NLM
STAT- MEDLINE
DCOM- 19990623
LR  - 20220310
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 66
IP  - 1
DP  - 1999 Apr
TI  - Trisomy 8 in stage I and stage III ovarian cancer detected by fluorescence in 
      situ hybridization.
PG  - 76-81
AB  - Ovarian cancer is the leading cause of death from gynecologic maligancy among 
      women in the United States. In 1997, there were nearly 27,000 ovarian cancer 
      cases with over 14,000 deaths. Recent attempts at early detection of ovarian 
      cancer have been aimed at the identification of biomarkers that would indicate an 
      underlining malignant process or reflect the biological behavior of the tumor. 
      Our previous studies revealed that chromosome 8 copy number abnormality, 
      especially trisomy, is common in several cancers. Archival tissues from 24 cases 
      of papillary serous ovarian carcinoma (10 stage I and 14 stage III) were analyzed 
      by fluorescence in situ hybridization (FISH) with a chromosome 8-specific 
      alpha-satellite probe (Oncor, Gaithersburg, MD). The analysis was done according 
      to standard protocols of the Lifespan Academic Medical Center Cytogenetics 
      Laboratory at Rhode Island Hospital. Twenty-one of 24 cases (87.5%) were found to 
      be trisomic for chromosome 8, if a cutoff point of >/=15% cells with three 
      signals is adopted. Overall, 80% of stage I and 93% of stage III tumors had 
      trisomy 8. This study confirms the presence of a high frequency of trisomy 8 in 
      both early and late stages of the disease and suggests that trisomy 8 may be an 
      early event in the multistep process leading to ovarian cancer. It is of interest 
      to note that a higher frequency of trisomy 8 was found in a higher stage of 
      disease, consistent with our previous results on breast cancer. Thus, additional 
      FISH studies of ovarian tumors for chromosome 8 copy number assessment may be 
      warranted.
CI  - Copyright 1999 Academic Press.
FAU - Mark, H F
AU  - Mark HF
AD  - Lifespan Academic Medical Center Cytogenetics Laboratory, Rhode Island Hospital 
      and Brown University School of Medicine, Providence, Rhode Island 02903, USA.
FAU - Afify, A M
AU  - Afify AM
FAU - Werness, B A
AU  - Werness BA
FAU - Das, S
AU  - Das S
FAU - Mark, S
AU  - Mark S
FAU - Samy, M
AU  - Samy M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 8
MH  - Cystadenocarcinoma, Papillary/*genetics/*pathology/surgery
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Ovarian Neoplasms/*genetics/*pathology/surgery
MH  - Retrospective Studies
MH  - *Trisomy
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - S0014-4800(99)92241-4 [pii]
AID - 10.1006/exmp.1999.2241 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 1999 Apr;66(1):76-81. doi: 10.1006/exmp.1999.2241.