PMID- 10332832
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20181201
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 36
IP  - 3
DP  - 1999 May
TI  - Helicobacter felis infection in dogs: effect on gastric structure and function.
PG  - 237-48
AB  - The relationship of Helicobacter felis, an organism that is observed in the 
      stomachs of dogs, to gastric disease in dogs is unclear. The objective of this 
      study was to determine if Helicobacter felis infection alters gastric morphology 
      and gastric secretory function in dogs. Five specific-pathogen-free (SPF), 
      Helicobacter-free Beagle dogs were examined before and for 26 weeks after 
      inoculation with H. felis (ATCC 49179). Three SPF uninfected dogs served as 
      controls. All five dogs became colonized by H. felis as determined by urease 
      activity, histopathology, polymerase chain reaction, and transmission electron 
      microscopic examination of serial gastric biopsies. The degree of colonization 
      ranged from < 1 organism/400 x field to > 10 organisms/400 x field. The fundus, 
      body, and cardia were most heavily colonized. Evaluation of gastric biopsies 
      showed mild gastric inflammation and lymphoid follicles in both infected and 
      uninfected dogs. There was no correlation between the number of organisms 
      observed and the degree of gastric inflammation or number of lymphoid follicles. 
      The gastric secretory axis, assessed by fasting and meal-stimulated plasma 
      gastrin, mucosal gastrin and somatostatin immunoreactivity, fasting gastric pH, 
      and pentagastrin-stimulated gastric acid secretion, was similar in both infected 
      and uninfected dogs. Fasting gastric pH was not a reliable indicator of gastric 
      secretory function. These findings suggest that H. felis may not be a gastric 
      pathogen in dogs. However, the density of colonization and limited duration of 
      infection should be considered when interpreting these findings.
FAU - Simpson, K W
AU  - Simpson KW
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Cornell 
      University, Ithaca, NY, USA. KWS5@cornell.edu
FAU - McDonough, P L
AU  - McDonough PL
FAU - Strauss-Ayali, D
AU  - Strauss-Ayali D
FAU - Chang, Y F
AU  - Chang YF
FAU - Harpending, P
AU  - Harpending P
FAU - Valentine, B A
AU  - Valentine BA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Gastrins)
RN  - 51110-01-1 (Somatostatin)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Animals
MH  - Biopsy/veterinary
MH  - Blotting, Southern/veterinary
MH  - DNA Primers/chemistry
MH  - DNA, Bacterial/chemistry
MH  - Dog Diseases/*microbiology/pathology/physiopathology
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Gastric Acid/metabolism
MH  - Gastrins/analysis/blood
MH  - Helicobacter/*pathogenicity
MH  - Helicobacter Infections/pathology/physiopathology/*veterinary
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Electron/veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Radioimmunoassay/veterinary
MH  - Somatostatin/analysis
MH  - Specific Pathogen-Free Organisms
MH  - Stomach/*microbiology/pathology/physiopathology
MH  - Stomach Diseases/pathology/physiopathology/*veterinary
MH  - Urease/analysis
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - 10.1354/vp.36-3-237 [doi]
PST - ppublish
SO  - Vet Pathol. 1999 May;36(3):237-48. doi: 10.1354/vp.36-3-237.