PMID- 10332958
OWN - NLM
STAT- MEDLINE
DCOM- 19990824
LR  - 20091119
IS  - 0040-3709 (Print)
IS  - 0040-3709 (Linking)
VI  - 59
IP  - 5
DP  - 1999 May
TI  - Screening of human placentas for chromosomal mosaicism using comparative genomic 
      hybridization.
PG  - 325-30
AB  - Detection of confined placental mosaicism (CPM) in term placental tissues is 
      usually accomplished by conventional cytogenetic analysis of cultured chorionic 
      stroma and direct preparations from trophoblast or, more recently, by 
      fluorescence in situ hybridization (FISH) on interphase nuclei. In this study, we 
      describe the use of comparative genomic hybridization (CGH) for detection of 
      chromosomal aneuploidy in term placentas and evaluate the sensitivity of this 
      novel approach for CPM diagnosis in multiple placental samples acquired from five 
      pregnancies prenatally diagnosed with CPM7 and CPM16. Each sample of placental 
      villi was separated enzymatically into trophoblast and chorionic stroma, and the 
      level of aneuploidy (three signals/nuclei) in each tissue was determined by FISH 
      analysis, using centromeric DNA probes specific for chromosome 7 (D7Z1/Z2) or 16 
      (D16Z2). Aneuploidy levels ranged from 5.2-96.1% in the 11 tissues with CPM7 and 
      9.8-93% in the 29 tissues with CPM16. Subsequently, CGH analysis of DNA from the 
      trophoblast and chorionic stroma of the same tissue sites detected the trisomic 
      clone in all placental tissues with aneuploidy (16%, as determined by FISH 
      analysis). Our results demonstrate the sensitivity of CGH analysis for detection 
      of chromosomal aneuploidy mosaicism and support our contention that the CGH 
      technique is the most effective cytogenetic method for screening term placentas 
      for the presence of CPM.
FAU - Lestou, V S
AU  - Lestou VS
AD  - Department of Pathology and Laboratory Medicine, University of British Columbia 
      and British Columbia Research Institute for Children's and Women's Health, 
      Vancouver, Canada.
FAU - Lomax, B L
AU  - Lomax BL
FAU - Barrett, I J
AU  - Barrett IJ
FAU - Kalousek, D K
AU  - Kalousek DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Teratology
JT  - Teratology
JID - 0153257
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chorionic Villi/chemistry
MH  - Chromosomes, Human, Pair 16
MH  - Chromosomes, Human, Pair 7
MH  - DNA/isolation & purification
MH  - Female
MH  - Genetic Testing/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - Mosaicism/*diagnosis/genetics
MH  - Nucleic Acid Hybridization/*methods
MH  - Placenta/chemistry
MH  - Pregnancy
MH  - Prenatal Diagnosis
MH  - Sensitivity and Specificity
MH  - Trisomy/diagnosis
EDAT- 1999/05/20 06:00
MHDA- 2000/06/20 09:00
CRDT- 1999/05/20 06:00
PHST- 1999/05/20 06:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/20 06:00 [entrez]
AID - 10.1002/(SICI)1096-9926(199905)59:5<325::AID-TERA3>3.0.CO;2-N [pii]
AID - 10.1002/(SICI)1096-9926(199905)59:5<325::AID-TERA3>3.0.CO;2-N [doi]
PST - ppublish
SO  - Teratology. 1999 May;59(5):325-30. doi: 
      10.1002/(SICI)1096-9926(199905)59:5<325::AID-TERA3>3.0.CO;2-N.