PMID- 10332965
OWN - NLM
STAT- MEDLINE
DCOM- 19990630
LR  - 20171116
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 26
IP  - 5
DP  - 1999 May
TI  - Ligation of CD40 induced tumor necrosis factor-alpha in rheumatoid arthritis: a 
      novel mechanism of activation of synoviocytes.
PG  - 1035-43
AB  - OBJECTIVE: To determine the immunopathological significance of CD40/CD40 ligand 
      (CD40L) interaction in rheumatoid arthritis (RA). METHODS: The expression of CD40 
      ligand (CD40L) in synovial tissues (ST) from patients with RA was examined by 
      immunohistochemistry. Tumor necrosis factor-alpha (TNF-alpha) was measured by 
      ELISA. Expression of CD40 on ST cells was quantified by anti-CD40 monoclonal 
      antibodies and 125I labelled anti-mouse IgG. RESULTS: Immunohistochemistry showed 
      CD40L+ T cells in RA ST. Ligation of CD40 on RA ST cells significantly increased 
      the production of TNF-alpha in a dose dependent fashion. Adherent, but not 
      non-adherent, fraction of ST cells responded to ligation of CD40 to produce 
      TNF-alpha. Interferon-gamma (IFN-gamma), interleukin 4 (IL-4), or IL-13 acted 
      synergistically with CD40 ligation to enhance TNF-alpha production by ST cells. 
      IL-10 exerted inhibitory effects on both CD40 ligation induced and CD40 ligation 
      plus IFN-gamma induced TNF-alpha production by ST cells. CONCLUSION: These data 
      indicate activated T cells participate in synovial inflammation of RA via CD40L 
      to stimulate the production of TNF-alpha by ST cells. The effect of CD40 ligation 
      is modulated by the presence of several cytokines, e.g., IFN-gamma, IL-4, IL-10, 
      and IL-13.
FAU - Harigai, M
AU  - Harigai M
AD  - Institute of Rheumatology, Tokyo Women's Medical University, Japan.
FAU - Hara, M
AU  - Hara M
FAU - Nakazawa, S
AU  - Nakazawa S
FAU - Fukasawa, C
AU  - Fukasawa C
FAU - Ohta, S
AU  - Ohta S
FAU - Sugiura, T
AU  - Sugiura T
FAU - Inoue, K
AU  - Inoue K
FAU - Kashiwazaki, S
AU  - Kashiwazaki S
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Arthritis, Rheumatoid/immunology/*metabolism/pathology
MH  - *CD40 Antigens/metabolism
MH  - CD40 Ligand
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis/immunology
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Lymphocyte Activation
MH  - Membrane Glycoproteins/biosynthesis/*metabolism
MH  - Synovial Membrane/metabolism/*pathology
MH  - T-Lymphocytes/immunology/pathology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/*metabolism
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1999 May;26(5):1035-43.