PMID- 10333359
OWN - NLM
STAT- MEDLINE
DCOM- 19990723
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 5
IP  - 3
DP  - 1999 Mar
TI  - Inverse relationship between apoptosis and Bcl-2 expression in 
      syncytiotrophoblast and fibrin-type fibrinoid in early gestation.
PG  - 246-51
AB  - The purpose of this study was to assess the role of apoptosis and cell cycle 
      arrest in the trophoblast during early gestation by determining the location of 
      apoptotic cells and examining the expression of Bcl-2 and p21. Using the terminal 
      deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) 
      method on human chorionic villi, a cluster of apoptotic nuclei was demonstrated 
      in perivillous fibrin-type fibrinoid, but no apoptotic changes were identified in 
      the syncytiotrophoblast or in other subtypes of the trophoblast. The 
      syncytiotrophoblast was diffusely positive for Bcl-2, but fibrin-type fibrinoid 
      was negative for Bcl-2. Hence, there was an inverse relationship between 
      apoptosis and Bcl-2 expression in both fibrin-type fibrinoid and 
      syncytiotrophoblast. Expression of p21 was present to some extent in the 
      syncytiotrophoblast, but not in fibrin-type fibrinoid. These results suggest that 
      Bcl-2 may play an important role in preventing apoptosis in the 
      syncytiotrophoblast; this may be necessary to prevent any DNA degradation from 
      being spread to other nuclei in a multinuclear cell like the syncytiotrophoblast.
FAU - Toki, T
AU  - Toki T
AD  - Department of Obstetrics and Gynecology, Shinshu University School of Medicine, 
      Matsumoto, Japan.
FAU - Horiuchi, A
AU  - Horiuchi A
FAU - Ichikawa, N
AU  - Ichikawa N
FAU - Mori, A
AU  - Mori A
FAU - Nikaido, T
AU  - Nikaido T
FAU - Fujii, S
AU  - Fujii S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/metabolism
MH  - DNA Fragmentation
MH  - Female
MH  - Fibrin/*metabolism
MH  - *Gestational Age
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Trophoblasts/*cytology/*metabolism
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - 10.1093/molehr/5.3.246 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 1999 Mar;5(3):246-51. doi: 10.1093/molehr/5.3.246.