PMID- 10333532
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 441
IP  - 2
DP  - 1999 May 17
TI  - Griseofulvin-induced aneuploidy and meiotic delay in male mouse germ cells: 
      detected by using conventional cytogenetics and three-color FISH.
PG  - 181-90
AB  - Griseofulvin (GF) was tested in male mouse germ cells for the induction of 
      meiotic delay and aneuploidy. Starved mice were orally treated with 500, 1000 and 
      2000 mg/kg of GF in corn oil and testes were sampled 22 h later for meiotic delay 
      analysis and chromosome counting in spermatocytes at the second meiotic metaphase 
      (MMII). A dose-related increase in meiotic delay by dose-dependently arresting 
      spermatocytes in first meiotic metaphase (MMI) or/and prolonging interkinesis was 
      observed. Hyperhaploid MMII cells were not significantly increased. Sperm were 
      sampled from the Caudae epididymes 22 days after GF-treatment of the males for 
      three-color fluorescence in situ hybridization (FISH). The frequencies of 
      diploidies were 0.01-0.02% in sperm of the solvent control animals and increased 
      dose-dependently to 0.03%, 0.068% and 0.091%, respectively, for 500, 1000 and 
      2000 mg/kg of GF. The frequencies of disomic sperm were increased significantly 
      above the controls in all GF-treated groups but showed no dose response. The data 
      for individual classes of disomic sperm indicated that MII was more sensitive 
      than MI to GF-induced non-disjunction in male mice. A comparison of the present 
      data from male mice and literature data from female mice suggests that mouse 
      oocytes are more sensitive than mouse spermatocytes to GF-induced meiotic delay 
      and aneuploidy.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Shi, Q
AU  - Shi Q
AD  - Institut fuer Saeugetiergenetik, GSF Forschungszentrum fuer Umwelt und 
      Gesundheit, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany.
FAU - Schmid, T E
AU  - Schmid TE
FAU - Adler, I
AU  - Adler I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antifungal Agents)
RN  - 0 (DNA Probes)
RN  - 32HRV3E3D5 (Griseofulvin)
SB  - IM
MH  - Aneuploidy
MH  - Animals
MH  - Antifungal Agents/*pharmacology
MH  - Chromosome Aberrations
MH  - DNA Probes/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Epididymis/drug effects
MH  - Female
MH  - Griseofulvin/*pharmacology
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Male
MH  - Meiosis/*drug effects
MH  - Mice
MH  - Microscopy, Fluorescence/veterinary
MH  - Random Allocation
MH  - Spermatozoa/*drug effects
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - S1383571899000467 [pii]
AID - 10.1016/s1383-5718(99)00046-7 [doi]
PST - ppublish
SO  - Mutat Res. 1999 May 17;441(2):181-90. doi: 10.1016/s1383-5718(99)00046-7.