PMID- 10336892
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20190516
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 13
IP  - 9
DP  - 1999 Jun
TI  - Differentiation lineage-specific expression of human heat shock transcription 
      factor 2.
PG  - 1089-98
AB  - Differentiation of multipotential hematopoietic cells into lineage-committed 
      precursors involves the selection and maintenance of appropriate programs of gene 
      expression, regulated by specific transcription factors. Using human K562 
      erythroleukemia cells capable of differentiating along erythroid and 
      megakaryocytic lineages, we explore the differentiation-related role of heat 
      shock transcription factor 2 (HSF2), which belongs to a family of transcription 
      factors generally known to regulate heat shock gene expression. We demonstrate 
      that enhanced HSF2 expression and the acquisition of HSF2 DNA binding activity 
      are strictly specific for erythroid characteristics of K562 cells. Our results 
      reveal a multistep regulatory process of HSF2 gene expression. In K562 cells 
      undergoing hemin-mediated erythroid differentiation, the increase in HSF2 protein 
      levels is preceded by transcriptional induction of the HSF2 gene, accompanied by 
      increased HSF2 mRNA stability. In contrast, during megakaryocytic differentiation 
      induced by the phorbol ester TPA, expression of HSF2 is rapidly down-regulated, 
      leading to a complete loss of the HSF2 protein. These results indicate that the 
      determination of HSF2 expression occurs at the early stages of lineage 
      commitment. Taken together, our data suggest that HSF2 could function as a 
      lineage-restricted transcription factor during differentiation of K562 cells 
      along either the erythroid or the megakaryocytic pathway.
FAU - Pirkkala, L
AU  - Pirkkala L
AD  - Turku Centre for Biotechnology, Department of Biology, Anatomy, and Biochemistry 
      and Pharmacy, Abo Akademi University, University of Turku, FIN-20521 Turku, 
      Finland.
FAU - Alastalo, T P
AU  - Alastalo TP
FAU - Nykanen, P
AU  - Nykanen P
FAU - Seppa, L
AU  - Seppa L
FAU - Sistonen, L
AU  - Sistonen L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Transcription Factors)
RN  - 142297-91-4 (HSF2 protein, human)
RN  - 743LRP9S7N (Hemin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - *Erythropoiesis
MH  - *Gene Expression Regulation, Developmental
MH  - Heat-Shock Proteins/*biosynthesis
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Hemin/pharmacology
MH  - Humans
MH  - Leukemia, Erythroblastic, Acute
MH  - Megakaryocytes/cytology
MH  - Promoter Regions, Genetic
MH  - RNA Processing, Post-Transcriptional
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription Factors/*biosynthesis
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1999/05/25 00:00
MHDA- 1999/05/25 00:01
CRDT- 1999/05/25 00:00
PHST- 1999/05/25 00:00 [pubmed]
PHST- 1999/05/25 00:01 [medline]
PHST- 1999/05/25 00:00 [entrez]
AID - 10.1096/fasebj.13.9.1089 [doi]
PST - ppublish
SO  - FASEB J. 1999 Jun;13(9):1089-98. doi: 10.1096/fasebj.13.9.1089.