PMID- 10337619
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20220316
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 5
DP  - 1999 May
TI  - Genomic organization of the rat alpha 2u-globulin gene cluster.
PG  - 463-70
AB  - The alpha 2u-globulin are a group of similar proteins, belonging to the lipocalin 
      superfamily of proteins, that are synthesized in a subset of secretory tissues in 
      rats. The many alpha 2u-globulin isoforms are encoded by a multigene family that 
      exhibits extensive homology. Despite a high degree of sequence identity, 
      individual family members show diverse expression patterns involving complex 
      hormonal, tissue-specific, and developmental regulation. Analysis suggests that 
      there are approximately 20 alpha 2u-globulin genes in the rat genome. We have 
      used fluorescence in situ hybridization (FISH) to show that the alpha 2u-globulin 
      genes are clustered at a single site on rat Chromosome (Chr) 5 (5q22-24). 
      Southern blots of rat genomic DNA separated by pulsed field gel electrophoresis 
      indicated that the alpha 2u-globulin genes are contained on two NruI fragments 
      with a total size of 880 kbp. Analysis of three P1 clones containing alpha 
      2u-globulin genes indicated that the alpha 2u-globulin genes are tandemly 
      arranged in a head-to-tail fashion. The organization of the alpha 2u-globulin 
      genes in the rat as a tandem array of single genes differs from the homologous 
      major urinary protein genes in the mouse, which are organized as tandem arrays of 
      divergently oriented gene pairs. The structure of these gene clusters may have 
      consequences for the proposed function, as a pheromone transporter, for the 
      protein products encoded by these genes.
FAU - McFadyen, D A
AU  - McFadyen DA
AD  - Department of Biological Sciences, University of Alberta, Edmonton, Canada.
FAU - Addison, W
AU  - Addison W
FAU - Locke, J
AU  - Locke J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (Alpha-Globulins)
RN  - 0 (DNA Primers)
RN  - 0 (alpha(2)-microglobulin)
SB  - IM
MH  - Alpha-Globulins/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - In Situ Hybridization, Fluorescence
MH  - *Multigene Family
MH  - Rats
MH  - Restriction Mapping
EDAT- 1999/05/25 00:00
MHDA- 1999/05/25 00:01
CRDT- 1999/05/25 00:00
PHST- 1999/05/25 00:00 [pubmed]
PHST- 1999/05/25 00:01 [medline]
PHST- 1999/05/25 00:00 [entrez]
AID - 10.1007/s003359901024 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 May;10(5):463-70. doi: 10.1007/s003359901024.