PMID- 10337971
OWN - NLM
STAT- MEDLINE
DCOM- 19990915
LR  - 20071115
IS  - 0192-9763 (Print)
IS  - 0192-9763 (Linking)
VI  - 20
IP  - 3
DP  - 1999 May
TI  - The protective effect of brain-derived neurotrophic factor after gentamicin 
      ototoxicity.
PG  - 317-24
AB  - HYPOTHESIS: Brain-derived neurotrophic factor (BDNF) influences the process of 
      hair cell recovery in the vestibular sensory epithelium of the chinchilla after 
      local application of gentamicin (GM). BACKGROUND: Hair cell regeneration in the 
      inner ear after GM ototoxicity has been demonstrated. However, the mechanisms 
      responsible for this recovery have yet to be completely elucidated. This report 
      examines the protective and proliferative effects that BDNF exerts on vestibular 
      hair cells in experiments designed to further elucidate the mechanisms of hair 
      cell regeneration. METHODS: The inner ears of three separate groups of 
      chinchillas were treated with GM only, GM and BDNF simultaneously, and GM 
      followed by BDNF 1 week later. The numbers of hair and supporting cells in the 
      horizontal cristae of each group were then estimated at 1, 2, 4, and 8 weeks, and 
      the data were compared. RESULTS: Type I hair cells after GM treatment completely 
      disappeared. After simultaneous BDNF and GM treatment, their numbers decreased to 
      23% at 1 week and progressively disappeared by week 8. When BDNF was applied 1 
      week after GM administration, type I hair cells recovered to 12% at week 4 and 
      28% at week 8. Type II hair cells after GM treatment decreased to 15%, but 
      recovered to 83% 4 weeks later. Simultaneous administration of BDNF and GM 
      prevented the ototoxic effects of GM alone. When BDNF was administered 1 week 
      after GM, type II hair cell recovery was accelerated and was greater than after 
      GM alone (81% versus 18%). Supporting cells after GM treatment decreased to 74% 
      at 1 week after treatment, recovered to 91% at 2 weeks, and remained at 86% at 4 
      weeks and 85% at 8 weeks. With the simultaneous administration of BDNF and GM, 
      supporting cells significantly decreased at 2 weeks after treatment (63%), but 
      recovered to normal by week 8. CONCLUSIONS: These results suggest that BDNF 
      provided simultaneously with GM minimizes the ototoxic effect of GM on type II 
      hair cells. The increase in the number of new hair cells when BDNF is provided 
      after ototoxic damage is evidence of the proliferative capacity of this 
      neurotrophic factor.
FAU - Lopez, I
AU  - Lopez I
AD  - Victor Goodhill Ear Center, Division of Head and Neck Surgery, School of 
      Medicine, University of California, Los Angeles 90095-1624, USA.
FAU - Honrubia, V
AU  - Honrubia V
FAU - Lee, S C
AU  - Lee SC
FAU - Chung, W H
AU  - Chung WH
FAU - Li, G
AU  - Li G
FAU - Beykirch, K
AU  - Beykirch K
FAU - Micevych, P
AU  - Micevych P
LA  - eng
GR  - DC01404/DC/NIDCD NIH HHS/United States
GR  - DC02952/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Otol
JT  - The American journal of otology
JID - 7909513
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gentamicins)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*adverse effects
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Chinchilla
MH  - Gentamicins/*adverse effects
MH  - Hair Cells, Auditory/*drug effects/pathology/*physiology
MH  - Male
MH  - Regeneration/drug effects/*physiology
MH  - Time Factors
EDAT- 1999/05/25 00:00
MHDA- 1999/05/25 00:01
CRDT- 1999/05/25 00:00
PHST- 1999/05/25 00:00 [pubmed]
PHST- 1999/05/25 00:01 [medline]
PHST- 1999/05/25 00:00 [entrez]
PST - ppublish
SO  - Am J Otol. 1999 May;20(3):317-24.