PMID- 10339452
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 107
IP  - 6
DP  - 1999 Jun
TI  - Cytogenetic effects from exposure to mixed pesticides and the influence from 
      genetic susceptibility.
PG  - 501-5
AB  - Exposure to pesticides remains a major environmental health problem. Health risk 
      from such exposure needs to be more precisely understood. We conducted three 
      different cytogenetic assays to elucidate the biological effects of exposure to 
      mixed pesticides in 20 Costa Rica farmers (all nonsmokers) compared with 20 
      matched controls. The farmers were also exposed to dibromochloropropane during 
      the early employment years, and most of them experienced sterility/fertility 
      problems. Our data show that the farmers had consistently higher frequencies of 
      chromosome aberrations, as determined by the standard chromosome aberration 
      assay, and significantly abnormal DNA repair responses (p < 0.05), as determined 
      by the challenge assay, but no statistically significant differences in the 
      tandem-probe fluorescence in situ hybridization (FISH) assay (p > 0.05). Genotype 
      analysis indicates that farmers with certain "unfavorable" versions of 
      polymorphic metabolizing genes (cytochrome P4502E1, the glutathione 
      S-transferases mu and theta, and the paraoxonase genes) had significantly more 
      biological effects, as determined by all three cytogenetic assays, than both the 
      farmers with the "favorable" alleles and the matched controls. A unique 
      observation is that, in individuals who had inherited any of the mentioned 
      "unfavorable" alleles, farmers were consistently underrepresented. In conclusion, 
      the Costa Rican farmers were exposed to genotoxic agents, most likely pesticides, 
      which expressed the induction of biological and adverse health effects. The 
      farmers who had inherited "unfavorable" metabolizing alleles were more 
      susceptible to genotoxic effects than those with "favorable" alleles. Our 
      genotype data suggest that the well-recognized "healthy worker effect" may be 
      influenced by unrecognized occupational selection pressure against genetically 
      susceptible individuals.
FAU - Au, W W
AU  - Au WW
AD  - Department of Preventive Medicine and Community Health, The University of Texas 
      Medical Branch, Galveston, Texas 77555-1110, USA. william.au@utmb.edu
FAU - Sierra-Torres, C H
AU  - Sierra-Torres CH
FAU - Cajas-Salazar, N
AU  - Cajas-Salazar N
FAU - Shipp, B K
AU  - Shipp BK
FAU - Legator, M S
AU  - Legator MS
LA  - eng
GR  - P30 ES 06676/ES/NIEHS NIH HHS/United States
GR  - T32 ES 07254/ES/NIEHS NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Mutagens)
RN  - 0 (Pesticides)
SB  - IM
MH  - Adult
MH  - Agricultural Workers' Diseases/*chemically induced/*genetics
MH  - Alleles
MH  - Chromosome Aberrations/genetics
MH  - Costa Rica
MH  - DNA Repair/drug effects
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mutagenicity Tests
MH  - Mutagens/*toxicity
MH  - Pesticides/*toxicity
MH  - Polymorphism, Restriction Fragment Length
PMC - PMC1566563
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
PMCR- 1999/06/01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PHST- 1999/06/01 00:00 [pmc-release]
AID - sc271_5_1835 [pii]
AID - 10.1289/ehp.99107501 [doi]
PST - ppublish
SO  - Environ Health Perspect. 1999 Jun;107(6):501-5. doi: 10.1289/ehp.99107501.