PMID- 10339970
OWN - NLM
STAT- MEDLINE
DCOM- 19990611
LR  - 20151008
IS  - 0029-0203 (Print)
IS  - 0029-0203 (Linking)
VI  - 103
IP  - 4
DP  - 1999 Apr
TI  - [Expression of brain-derived neurotrophic factor gene in retina following 
      vitreous tap].
PG  - 271-6
AB  - PURPOSE: Mechanical injuries to the retina following vitreous tap are reported to 
      protect photoreceptor cells in a rat model of the retinal degeneration and 
      enhance the survival rate of retinal ganglion cells in the optic nerve 
      transection. Neurotrophic factors are presumably involved in the protective 
      mechanisms. In order to see whether neurotrophic factors are synthesized in the 
      retina, we studied the expression of neurotrophic factors in the retina following 
      vitreous tap in rats. MATERIALS AND METHODS: One eye each of 20 mature rats 
      received transscleral vitreous taps at three points of entry and retinal injury. 
      The retinas were removed and examined at day 0 to 14 of treatment. RESULTS: 
      Following injury, the retina showed increased expression of glial fibrillary 
      acidic protein (GFAP) mRNA and brain-derived neurotrophic factor (BDNF) mRNA. 
      There was no enhancement of neurotrophin-3 (NT-3) mRNA when examined by reverse 
      transcription-polymerase chain reaction (RT-PCR). CONCLUSIONS: Activated retinal 
      glial cells may produce BDNF which prevents retinal neuronal cell damage 
      following injury.
FAU - Sakai, T
AU  - Sakai T
AD  - Department of Ophthalmology, Jikei University School of Medicine, Japan.
FAU - Yoshitoshi, T
AU  - Yoshitoshi T
FAU - Kawagoe, M
AU  - Kawagoe M
FAU - Mizobuchi, T
AU  - Mizobuchi T
FAU - Kitahara, K
AU  - Kitahara K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nippon Ganka Gakkai Zasshi
JT  - Nippon Ganka Gakkai zasshi
JID - 7505716
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Glial Fibrillary Acidic Protein/genetics
MH  - Male
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/injuries/*metabolism
MH  - Vitreous Body/physiology
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PST - ppublish
SO  - Nippon Ganka Gakkai Zasshi. 1999 Apr;103(4):271-6.