PMID- 10340441
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 282
IP  - 1-2
DP  - 1999 Apr
TI  - An inhibitor-free assay of acetylcholinesterase and butyrylcholinesterase in the 
      cerebrospinal fluid.
PG  - 135-45
AB  - An inhibitor-free assay for the simultaneous determination of 
      acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in the cerebrospinal 
      fluid (CSF) is described. It is based on our finding that the individual activity 
      ratios of BChE on both its substrates acetylthiocholine (ACh) and 
      butyrylthiocholine (BCh) in the CSF and in the parallel serum are identical under 
      conditions of at least 5 mmol/l substrate concentration (Q(BChE)SE = Q(BChe)CSF). 
      Considering that AChE only reacts with ACh as substrate and occurs with 
      negligible activities in the serum, the measured individual activity ratio of 
      BChE in the serum (Q(BChE)SE) and the total hydrolysis rate of ACh and BCh in the 
      CSF do allow a precise calculation of the AChE activity in the cerebrospinal 
      fluid. The derivation of the corresponding formula is demonstrated in detail. The 
      inhibitor-free assay was compared with procedures using cholinesterase inhibitors 
      (BW284c51 for AChE and/or iso-OMPA for BChE). Achieving widely identical results 
      in particular between the procedure using the AChE inhibitor and the 
      inhibitor-free test, the latter has decisive advantages: (1) it avoids the use of 
      highly toxic inhibitors, (2) it minimizes the test volume needed, (3) it 
      characterizes additionally the status of the blood-CSF barrier by means of the 
      BChE activity ratio in the CSF and in the parallel serum.
FAU - Kluge, H H
AU  - Kluge HH
AD  - Department of Neurochemistry, Clinic of Neurology, Friedrich Schiller-University, 
      Jena, Germany.
FAU - Kluge, W H
AU  - Kluge WH
FAU - Hartmann, W
AU  - Hartmann W
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Cholinesterase Inhibitors)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - EC 3.1.1.8 (Butyrylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/*cerebrospinal fluid
MH  - Butyrylcholinesterase/*cerebrospinal fluid
MH  - *Cholinesterase Inhibitors
MH  - Humans
MH  - Kinetics
MH  - Time Factors
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - S0009-8981(99)00021-2 [pii]
AID - 10.1016/s0009-8981(99)00021-2 [doi]
PST - ppublish
SO  - Clin Chim Acta. 1999 Apr;282(1-2):135-45. doi: 10.1016/s0009-8981(99)00021-2.