PMID- 10340820
OWN - NLM
STAT- MEDLINE
DCOM- 19990618
LR  - 20191024
VI  - 23
IP  - 4
DP  - 1999 Apr
TI  - Substrate utilization and thermogenic responses to beta-adrenergic stimulation in 
      obese subjects with NIDDM.
PG  - 411-8
AB  - OBJECTIVE: This study intended to investigate disturbances in 
      beta-adrenergically-mediated substrate utilization and thermogenesis in obese 
      subjects with mild non insulin-dependent diabetes mellitus (NIDDM). DESIGN: 
      Following a baseline period of 30 min, the beta-agonist isoproterenol (ISO) was 
      administered in increasing doses of 6, 12, and 24 ng/kgFFM x min, each dose for 
      30 min. SUBJECTS: Nine healthy lean males (CON, 50.6+/-2.4 y, % body fat: 
      16.0+/-1.8) and 10 obese subjects with NIDDM (51.8+/-2.4 y, % body fat 
      34.1+/-1.9). RESULTS: Basal non esterified fatty acid concentrations (NEFA) and 
      basal fat oxidation (absolute or expressed per unit fat free mass, FFM) were 
      significantly higher in NIDDM as compared to CON, whereas basal carbohydrate 
      (CHO) oxidation was significantly lower. The ISO-induced increase in 
      NEFA-concentrations was blunted in NIDDM (delta at 24 ng/kgFFM x min: CON: 
      717+/-59 micromol/l vs NIDDM: 358+/-97 micromol/l, P< 0.01). The non-protein 
      respiratory exchange ratio (RER) did not change in NIDDM and significantly 
      decreased in CON during ISO-infusion (P < 0.05), reflecting the tendency towards 
      a blunted increase in fat oxidation in NIDDM (delta fat ox at 24 ng; CON: 
      0.025+/-0.005 g/min vs NIDDM 0.016+/-0.007 g/min). The ISO-induced thermogenic 
      response was comparable in NIDDM and CON (at 24 ng %increase above baseline: CON: 
      16.8+/-2.2% vs NIDDM: 14.7+/-0.9%). At all time points, there were no significant 
      differences in circulating ISO and noradrenaline concentrations. Basal adrenaline 
      (A) concentrations and A concentrations during ISO-infusion were significantly 
      lower in NIDDM (basal A; CON: 64+/-15 pg/ml vs NIDDM: 25+/-2 pg/ml, P < 0.001). 
      CONCLUSION: There appear to be deviations in beta-adrenoceptor mediated fat 
      utilization and adrenal medulla function in obesity-associated NIDDM. The 
      impairments in sympathetically mediated fat utilization have previously been 
      observed in 'simple' obese subjects, indicating that these disturbances are 
      confined to the obese state per se.
FAU - Blaak, E E
AU  - Blaak EE
AD  - Department of Human Biology, Maastricht University, The Netherlands.
FAU - Saris, W H
AU  - Saris WH
FAU - Wolffenbuttel, B H
AU  - Wolffenbuttel BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Obes Relat Metab Disord
JT  - International journal of obesity and related metabolic disorders : journal of the 
      International Association for the Study of Obesity
JID - 9313169
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Insulin)
RN  - 0 (Receptors, Adrenergic, beta)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Adrenergic beta-Agonists
MH  - Blood Glucose/metabolism
MH  - Body Composition
MH  - *Body Temperature Regulation
MH  - Diabetes Mellitus/*metabolism
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Fatty Acids, Nonesterified/blood
MH  - Heart Rate
MH  - Humans
MH  - Insulin/blood
MH  - Isoproterenol
MH  - Male
MH  - Middle Aged
MH  - *Obesity
MH  - Oxygen Consumption
MH  - Pulmonary Gas Exchange
MH  - Receptors, Adrenergic, beta/*physiology
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1038/sj.ijo.0800837 [doi]
PST - ppublish
SO  - Int J Obes Relat Metab Disord. 1999 Apr;23(4):411-8. doi: 10.1038/sj.ijo.0800837.