PMID- 10341087
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 6
DP  - 1999 Jun
TI  - Construction and characterization of a porcine P1-derived artificial chromosome 
      (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six 
      type I and type II loci.
PG  - 569-72
AB  - A porcine P1-derived artificial chromosome (PAC) library of a male German 
      Landrace pig was constructed in pCYPAC2. In total 90,240 clones were generated 
      and individually transferred into microtiter plates. An average insert size of 
      119.1 kb was determined by analyzing 150 randomly selected PAC clones by pulsed 
      field electrophoresis, yielding approximately 3.2 genome equivalents. The 
      stability of nine clones was followed through 110 generations showing no 
      reduction of the insert size. The probability of identifying a specific 
      chromosomal region within the library was tested by screening for the presence of 
      seven type I and five type II loci. The analysis showed that most loci (10/12) 
      were present in the library at least twice. To determine the percentage of 
      chimerism, six clones were analyzed by fluorescence in situ hybridization (FISH) 
      on metaphase chromosomes. We assign one type I locus (Triadin) and three type II 
      loci (SW855, S0300, SW1129).
FAU - Al-Bayati, H K
AU  - Al-Bayati HK
AD  - Institute of Veterinary Medicine, University of Gottingen, Groner Landstrasse 2 
      37073 Goettingen, Germany.
FAU - Duscher, S
AU  - Duscher S
FAU - Kollers, S
AU  - Kollers S
FAU - Rettenberger, G
AU  - Rettenberger G
FAU - Fries, R
AU  - Fries R
FAU - Brenig, B
AU  - Brenig B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/*methods
MH  - Chromosomes
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - DNA Transposable Elements
MH  - Gene Library
MH  - Genetic Markers
MH  - Genome
MH  - Male
MH  - Physical Chromosome Mapping/methods
MH  - Polymerase Chain Reaction
MH  - Swine/*genetics
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1007/s003359901046 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Jun;10(6):569-72. doi: 10.1007/s003359901046.