PMID- 10341092
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 6
DP  - 1999 Jun
TI  - Isolation, genomic organization, and expression analysis of Men1, the murine 
      homolog of the MEN1 gene.
PG  - 592-6
AB  - The mouse homolog of the human MEN1 gene, which is defective in a dominant 
      familial cancer syndrome, multiple endocrine neoplasia type 1 (MEN1), has been 
      identified and characterized. The mouse Men1 transcript contains an open reading 
      frame encoding a protein of 611 amino acids which has 97% identity and 98% 
      similarity to human menin. Sequence of the entire Men1 gene (9.3 kb) was 
      assembled, revealing 10 exons, with exon 1 being non-coding; a polymorphic 
      tetranucleotide repeat was located in the 5'- flanking region. The exon-intron 
      organization and the size of the coding exons 2-9 were well conserved between the 
      human and mouse genes. Fluorescence in situ hybridization localized the Men1 gene 
      to mouse Chromosome (Chr) 19, a region known to be syntenic to human Chr 11q13, 
      the locus for the MEN1 gene. Northern analysis indicated two messages-2.7 kb and 
      3.1 kb-expressed in all stages of the embryo analyzed and in all eight adult 
      tissues tested. The larger transcript differs from the smaller by the inclusion 
      of an unspliced intron 1. Whole-mount in situ hybridization of 10.5-day and 
      11.5-day embryos showed ubiquitous expression of Men1 RNA. Western analysis with 
      antibodies raised against a conserved C-terminal peptide identified an 
      approximately 67-kDa protein in the lysates of adult mouse brain, kidney, liver, 
      pancreas, and spleen tissues, consistent with the size of human menin. The levels 
      of mouse menin do not appear to fluctuate during the cell cycle.
FAU - Guru, S C
AU  - Guru SC
AD  - Genetics and Molecular Biology Branch, National Human Genome Research Institute, 
      National Institutes of Health, Building 49, Room 3E-13, 49 Convent Drive, 
      Bethesda, Maryland, 20892-4442, USA.
FAU - Crabtree, J S
AU  - Crabtree JS
FAU - Brown, K D
AU  - Brown KD
FAU - Dunn, K J
AU  - Dunn KJ
FAU - Manickam, P
AU  - Manickam P
FAU - Prasad, N B
AU  - Prasad NB
FAU - Wangsa, D
AU  - Wangsa D
FAU - Burns, A L
AU  - Burns AL
FAU - Spiegel, A M
AU  - Spiegel AM
FAU - Marx, S J
AU  - Marx SJ
FAU - Pavan, W J
AU  - Pavan WJ
FAU - Collins, F S
AU  - Collins FS
FAU - Chandrasekharappa, S C
AU  - Chandrasekharappa SC
LA  - eng
SI  - GENBANK/AF109389
SI  - GENBANK/AF109390
PT  - Journal Article
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA, Complementary)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GCGGCCGC-specific type II deoxyribonucleases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Cycle/genetics
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Deoxyribonucleases, Type II Site-Specific/genetics
MH  - Embryo, Mammalian/physiology
MH  - Exons
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Situ Hybridization/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1007/s003359901051 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Jun;10(6):592-6. doi: 10.1007/s003359901051.