PMID- 10341177
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20220128
IS  - 1080-6040 (Print)
IS  - 1080-6059 (Electronic)
IS  - 1080-6040 (Linking)
VI  - 5
IP  - 3
DP  - 1999 May-Jun
TI  - Tuberculosis in the Caribbean: using spacer oligonucleotide typing to understand 
      strain origin and transmission.
PG  - 404-14
AB  - We used direct repeat (DR)-based spacer oligonucleotide typing (spoligotyping) 
      (in association with double-repetitive element polymerase chain reaction, 
      IS6110-restriction fragment length polymorphism [RFLP], and sometimes DR-RFLP and 
      polymorphic GC-rich sequence-RFLP) to detect epidemiologic links and transmission 
      patterns of Mycobacterium tuberculosis on Martinique, Guadeloupe, and French 
      Guiana. In more than a third of the 218 strains we typed from this region, 
      clusters and isolates shared genetic identity, which suggests epidemiologic 
      links. However, because of limited epidemiologic information, only 14.2% of the 
      strains could be directly linked. When spoligotyping patterns shared by two or 
      more isolates were pooled with 392 spoligotypes from other parts of the world, 
      new matches were detected, which suggests imported transmission. Persisting foci 
      of endemic disease and increased active transmission due to high population flux 
      and HIV-coinfection may be linked to the recent reemergence of tuberculosis in 
      the Caribbean. We also found that several distinct families of spoligotypes are 
      overrepresented in this region.
FAU - Sola, C
AU  - Sola C
AD  - Institut Pasteur de Guadeloupe, Pointe a Pitre.
FAU - Devallois, A
AU  - Devallois A
FAU - Horgen, L
AU  - Horgen L
FAU - Maisetti, J
AU  - Maisetti J
FAU - Filliol, I
AU  - Filliol I
FAU - Legrand, E
AU  - Legrand E
FAU - Rastogi, N
AU  - Rastogi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Emerg Infect Dis
JT  - Emerging infectious diseases
JID - 9508155
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - *Bacterial Typing Techniques
MH  - DNA Transposable Elements/genetics
MH  - DNA, Bacterial/analysis
MH  - French Guiana/epidemiology
MH  - Genetic Variation
MH  - Guadeloupe/epidemiology
MH  - Humans
MH  - Martinique/epidemiology
MH  - Mycobacterium tuberculosis/*classification/*genetics/isolation & purification
MH  - Oligonucleotides/analysis
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Restriction Fragment Length
MH  - Tuberculosis/*epidemiology/*microbiology/transmission
PMC - PMC2640778
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
PMCR- 1999/05/01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 10.3201/eid0503.990311 [doi]
PST - ppublish
SO  - Emerg Infect Dis. 1999 May-Jun;5(3):404-14. doi: 10.3201/eid0503.990311.