PMID- 10342572
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 33
IP  - 5-6
DP  - 1999 May
TI  - Fish analysis at diagnosis in acute lymphoblastic leukemia.
PG  - 441-9
AB  - Many recurrent cytogenetic abnormalities have been described in acute 
      lymphoblastic leukemia (ALL). With improvement of survival, it became evident 
      that some of these chromosomal changes had a major prognostic value: 
      hyperdiploidy and t(12;21) are associated with very long disease free survival, 
      whereas t(9;22) and 11q23 rearrangements correlate with a poor outcome. Because 
      of this prognostic impact, detection of these four specific abnormalities has to 
      be accurate and rapid at diagnosis. In order to reliably analyze ALL patients, we 
      used fluorescence in situ hybridization (FISH). We selected specific probes 
      enabling the detection of the structural rearrangements [t(9;22), t(12;21) and 
      11q23 rearrangements] directly on interphase cells. Detection of hyperdiploidy 
      was performed using comparative genomic hybridization (CGH). This technique 
      enables to characterize chromosomal gains and losses without requiring metaphase 
      obtention. We showed that this FISH-based approach was complementary to 
      conventional cytogenetics, and should be systematically used in ALL at diagnosis.
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
AD  - Laboratory of Haematology Biology Institute 9, Nantes, France.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 9
MH  - Gene Rearrangement
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis/genetics
MH  - Translocation, Genetic
RF  - 43
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.3109/10428199909058449 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 1999 May;33(5-6):441-9. doi: 10.3109/10428199909058449.