PMID- 10342733
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20191103
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 6
IP  - 4
DP  - 1998 Dec
TI  - Localization of C-X-C and C-C chemokines to renal tubular epithelial cells in 
      human kidney transplants is not confined to acute cellular rejection.
PG  - 203-8
AB  - Chemokines are important mediators of leucocyte chemoattraction to inflammatory 
      sites. Previous work has shown that the expression of some chemokines is 
      upregulated during renal transplant rejection. The objectives of the present 
      study were to determine whether chemokine expression is increased during renal 
      transplant rejection. Immunohistochemistry was used to localize the C-X-C (alpha) 
      chemokine interleukin-8 (IL-8) and the C-C (beta) chemokines monocyte 
      chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1beta 
      (MIP-1beta) in 30 needle biopsies of human kidney transplants taken for diagnosis 
      of renal dysfunction. Urine samples from transplant patients taken immediately 
      prior to biopsy were assayed for chemokine content using enzyme-linked 
      immunosorbent assays (ELISAs). Results from groups of patients having different 
      clinicopathological diagnoses were then compared. All three chemokines were 
      detected in most renal transplant biopsies showing acute cellular rejection but, 
      although infiltrating leucocytes were often positive, staining was predominantly 
      localized to renal tubular epithelium. Staining for MCP-1 was generally weaker 
      than for the other chemokines, and collecting tubules were usually stained more 
      strongly than proximal convoluted tubules. Tubular epithelial staining was also 
      found in biopsies from patients without signs of acute cellular rejection. There 
      were significantly higher amounts of IL-8 in the urine of patients with acute 
      cellular rejection, even when patients with urinary tract infections were 
      excluded, but mean titres of urinary MIP-1beta did not differ between patient 
      groups. This was also found when titres were normalized for urine volume and 
      creatinine levels. Production of IL-8, MCP-1 and MIP-1beta is not confined to 
      kidney transplants showing acute cellular rejection, and may be a relatively 
      nonspecific response of tubular epithelial cells to renal damage.
FAU - Sibbring, J S
AU  - Sibbring JS
AD  - Department of Immunology, Royal Liverpool University Hospital, UK.
FAU - Sharma, A
AU  - Sharma A
FAU - McDicken, I W
AU  - McDicken IW
FAU - Sells, R A
AU  - Sells RA
FAU - Christmas, S E
AU  - Christmas SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Interleukin-8)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Chemokine CCL2/*analysis/urine
MH  - Chemokine CCL4
MH  - Chemokines, CC/analysis/urine
MH  - Chemokines, CXC/analysis/urine
MH  - Epithelial Cells/chemistry
MH  - Female
MH  - Graft Rejection
MH  - Humans
MH  - Interleukin-8/*analysis/urine
MH  - Kidney Diseases/diagnosis/physiopathology/urine
MH  - Kidney Transplantation/*immunology
MH  - Kidney Tubules/*chemistry/pathology
MH  - Macrophage Inflammatory Proteins/*analysis/urine
MH  - Male
MH  - Middle Aged
MH  - Staining and Labeling
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - S0966-3274(98)80009-9 [pii]
AID - 10.1016/s0966-3274(98)80009-9 [doi]
PST - ppublish
SO  - Transpl Immunol. 1998 Dec;6(4):203-8. doi: 10.1016/s0966-3274(98)80009-9.