PMID- 10343615
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190501
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 52
IP  - 1
DP  - 1999 Jan
TI  - Human herpesvirus 6 latently infects mononuclear cells but not liver tissue.
PG  - 65-7
AB  - AIM: To investigate whether human herpesvirus 6 (HHV-6) can cause latent 
      infection of liver tissue. METHODS: Peripheral blood and liver tissue were 
      collected from 25 living related liver transplant recipients at the time of 
      transplantation. An avidin-biotin complex peroxidase method was used to identify 
      HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was 
      used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of 
      HHV-6 was determined by the presence of the Hind III site in a second PCR 
      product. RESULTS: Immunohistochemical analysis for HHV-6 antigen was negative in 
      all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA 
      was detected in peripheral blood mononuclear cells in nine of 25 recipients. All 
      nine HHV-6 identified in the mononuclear cells were variant B. CONCLUSIONS: HHV-6 
      variant B latently infects mononuclear cells but not liver tissue.
FAU - Yoshikawa, T
AU  - Yoshikawa T
AD  - Department of Paediatrics, Fujita Health University School of Medicine, Aichi, 
      Japan. tetsushi@fujita-hu.ac.jp
FAU - Suzuki, K
AU  - Suzuki K
FAU - Ihira, M
AU  - Ihira M
FAU - Furukawa, H
AU  - Furukawa H
FAU - Suga, S
AU  - Suga S
FAU - Iwasaki, T
AU  - Iwasaki T
FAU - Kurata, T
AU  - Kurata T
FAU - Asonuma, K
AU  - Asonuma K
FAU - Tanaka, K
AU  - Tanaka K
FAU - Asano, Y
AU  - Asano Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Viral/analysis
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/analysis
MH  - Female
MH  - Herpesviridae Infections/*diagnosis
MH  - Herpesvirus 6, Human/immunology/*isolation & purification/physiology
MH  - Humans
MH  - Infant
MH  - Leukocytes, Mononuclear/virology
MH  - Liver/*virology
MH  - *Liver Transplantation
MH  - Male
MH  - Virus Latency
PMC - PMC501010
EDAT- 1999/05/27 00:00
MHDA- 1999/05/27 00:01
PMCR- 2002/01/01
CRDT- 1999/05/27 00:00
PHST- 1999/05/27 00:00 [pubmed]
PHST- 1999/05/27 00:01 [medline]
PHST- 1999/05/27 00:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 10.1136/jcp.52.1.65 [doi]
PST - ppublish
SO  - J Clin Pathol. 1999 Jan;52(1):65-7. doi: 10.1136/jcp.52.1.65.