PMID- 10348186
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 726
IP  - 1-2
DP  - 1999 Apr 16
TI  - High-performance liquid chromatographic method for simultaneous determination of 
      [1-methyl-14C]caffeine and its eight major metabolites in rat urine.
PG  - 195-201
AB  - A selective and sensitive reversed-phase liquid chromatographic method was 
      developed for the simultaneous analysis of [1-Me-14C]caffeine and its eight major 
      radiolabelled metabolites in rat urine. The separation of the complex mixture of 
      caffeine metabolites was achieved by gradient elution with a dual solvent system 
      using an endcapped C18 reversed-phase column, which in contrast to commonly used 
      C18 reversed-phase columns also allows the separation of the two isomers of 
      6-amino-5-(N-formylmethylamino)-1,3-dimethyluracil (1,3,7-DAU), a caffeine 
      metabolite of quantitative importance predominantly occurring in rat. As caffeine 
      is metabolised primarily by members of the cytochrome P450 1A (CYP1A) subfamiliy, 
      determination of the pattern of caffeine metabolites in rat urine enables 
      analysis of activities of this important enzyme subfamily in vivo. Since CYP1A is 
      suggested to be involved in the detoxification of bilirubin, the assay may be 
      applied to search for untoxic inducers of CYP1A which might be of pharmacological 
      interest in the treatment of hyperbilirubinaemia.
FAU - Schrader, E
AU  - Schrader E
AD  - Department of Toxicology, Institute of Pharmacology and Toxicology, University of 
      Gottingen, Germany. eschrade@med.uni-goettingen.de
FAU - Klaunick, G
AU  - Klaunick G
FAU - Jorritsma, U
AU  - Jorritsma U
FAU - Neurath, H
AU  - Neurath H
FAU - Hirsch-Ernst, K I
AU  - Hirsch-Ernst KI
FAU - Kahl, G F
AU  - Kahl GF
FAU - Foth, H
AU  - Foth H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Carbon Radioisotopes)
RN  - 3G6A5W338E (Caffeine)
RN  - 77196-81-7 (1-methylcaffeine)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Animals
MH  - Caffeine/*analogs & derivatives/pharmacokinetics/urine
MH  - Carbon Radioisotopes
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Gunn
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet
EDAT- 1999/05/29 00:00
MHDA- 1999/05/29 00:01
CRDT- 1999/05/29 00:00
PHST- 1999/05/29 00:00 [pubmed]
PHST- 1999/05/29 00:01 [medline]
PHST- 1999/05/29 00:00 [entrez]
AID - 10.1016/s0378-4347(99)00040-7 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Apr 16;726(1-2):195-201. doi: 
      10.1016/s0378-4347(99)00040-7.