PMID- 10352239 OWN - NLM STAT- MEDLINE DCOM- 19990616 LR - 20091119 IS - 0022-1767 (Print) IS - 0022-1767 (Linking) VI - 162 IP - 11 DP - 1999 Jun 1 TI - Cutting edge: clonally restricted production of the neurotrophins brain-derived neurotrophic factor and neurotrophin-3 mRNA by human immune cells and Th1/Th2-polarized expression of their receptors. PG - 6303-6 AB - Neurotrophins, such as neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF), are potent regulators of neuronal functions. Here we show that human immune cells also produce NT-3 mRNA, secrete BDNF, and express their specific receptors trkB and trkC. The truncated trkB receptor, usually expressed in sensory neurons of the central nervous system, was also constitutively expressed in unstimulated Th cells. Full-length trkB was detectable in stimulated PBMC, B cell lines, and Th1, but not in Th2 and Th0 cell clones. Clonally restricted expression was also observed for trkC, until now not detected on blood cells. The Th1 cytokine IL-2 stimulated production of trkB mRNA but not of trkC, whereas the Th2 cytokine IL-4 enhanced NT-3 but not BDNF mRNA expression. Microbial Ags, which influence the Th1/Th2 balance, could therefore modulate the neurotrophic system and thereby affect neuronal synaptic activity of the central nervous system. FAU - Besser, M AU - Besser M AD - Institute of Immunology, University of Munich, Munich, Germany. FAU - Wank, R AU - Wank R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Immunol JT - Journal of immunology (Baltimore, Md. : 1950) JID - 2985117R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cytokines) RN - 0 (Nerve Growth Factors) RN - 0 (Neurotrophin 3) RN - 0 (RNA, Messenger) RN - 0 (Receptor, Ciliary Neurotrophic Factor) RN - 0 (Receptors, Nerve Growth Factor) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 2.7.10.1 (Receptor, trkC) SB - IM MH - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics/metabolism MH - Cells, Cultured MH - Clone Cells MH - Cytokines/physiology MH - Humans MH - Leukocytes, Mononuclear/*immunology/metabolism MH - Lymphocyte Activation MH - Nerve Growth Factors/*biosynthesis/genetics/metabolism MH - Neurotrophin 3 MH - RNA, Messenger/*biosynthesis MH - Receptor Protein-Tyrosine Kinases/*biosynthesis/genetics MH - Receptor, Ciliary Neurotrophic Factor MH - Receptor, trkB MH - Receptor, trkC MH - Receptors, Nerve Growth Factor/*biosynthesis/genetics MH - Th1 Cells/*metabolism MH - Th2 Cells/*metabolism EDAT- 1999/06/03 00:00 MHDA- 1999/06/03 00:01 CRDT- 1999/06/03 00:00 PHST- 1999/06/03 00:00 [pubmed] PHST- 1999/06/03 00:01 [medline] PHST- 1999/06/03 00:00 [entrez] PST - ppublish SO - J Immunol. 1999 Jun 1;162(11):6303-6.