PMID- 10352240
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20181201
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 11
DP  - 1999 Jun 1
TI  - Cutting edge: contrasting roles of TNF receptor-associated factor 2 (TRAF2) and 
      TRAF3 in CD40-activated B lymphocyte differentiation.
PG  - 6307-11
AB  - In B lymphocytes, CD40 signals contribute to the activation of Ab secretion, 
      isotype switching, T cell costimulation, and immunological memory. TRAF proteins 
      appear to be important components of the CD40 signal transduction complex, but 
      their roles in the activation of B cell effector functions are poorly understood. 
      We examined the contributions of TNF receptor-associated factor 2 (TRAF2) and 
      TRAF3 to CD40-activated differentiation in mouse B cells transfected with 
      inducible TRAF and dominant-negative TRAF cDNAs. We find that binding of TRAF2 
      and TRAF3 to CD40 is not required for the induction of Ab secretion, but that 
      both TRAF molecules can regulate the activation process. We demonstrate a 
      negative regulatory role for TRAF3 and that this activity is dependent on the 
      availability of an intact TRAF3-binding site in the cytoplasmic domain of CD40. 
      In contrast, TRAF2 appears to play a positive role in B cell differentiation, and 
      this activity is apparent even when its binding site on CD40 is disrupted.
FAU - Hostager, B S
AU  - Hostager BS
AD  - Department of Microbiology, University of Iowa, VA Medical Center, Iowa City 
      52242, USA.
FAU - Bishop, G A
AU  - Bishop GA
LA  - eng
GR  - AI28847/AI/NIAID NIH HHS/United States
GR  - CA66570/CA/NCI NIH HHS/United States
GR  - DK25295/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CD40 Antigens)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (TNF Receptor-Associated Factor 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Formation/genetics/immunology
MH  - B-Lymphocytes/*cytology/*immunology/metabolism
MH  - CD40 Antigens/genetics/metabolism/*physiology
MH  - Cell Differentiation/genetics/immunology
MH  - Cell Line
MH  - Humans
MH  - Lymphocyte Activation
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Binding/immunology
MH  - Protein Biosynthesis
MH  - Proteins/genetics/metabolism/*physiology
MH  - Receptors, Tumor Necrosis Factor/*physiology
MH  - TNF Receptor-Associated Factor 2
MH  - TNF Receptor-Associated Factor 3
MH  - Transfection/immunology
MH  - Tumor Cells, Cultured
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Jun 1;162(11):6307-11.