PMID- 10352357
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20181201
IS  - 0014-312X (Print)
IS  - 0014-312X (Linking)
VI  - 31
IP  - 3
DP  - 1999
TI  - Secretion of IL-6, monocyte chemoattractant protein-1, macrophage inflammatory 
      protein-1alpha, and TNFalpha by cultured intact human peritoneum.
PG  - 281-8
AB  - The peritoneum is an important site of host defence. The mesothelial cells, 
      lining the peritoneum, and the fibroblasts found in the layers below are potent 
      sources of a variety of mediators. Furthermore, granulocytes, mast cells, and 
      macrophages, either resident or attracted by inflammatory processes, are 
      interspersed within the tissue. We investigated the production of mediators by 
      samples of fresh human peritoneum. The method described here has the advantage 
      that the cellular composition of the human peritoneum remains intact. Samples of 
      peritoneum were excised at the beginning of elective abdominal operations in 
      infection-free patients. The tissue was placed across the wells of a microtitre 
      plate, fixed in place by the plate cover and incubated with culture medium with 
      or without lipopolysaccharide (LPS) for up to 5 h. The accumulation of IL-6, 
      monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory 
      protein-1alpha (MIP-1alpha) and TNFalpha in culture supernatants was measured by 
      ELISA. Production of MCP-1 and IL-6 occurred spontaneously during incubation and 
      was enhanced by as much as 4-fold in the presence of different concentrations of 
      LPS (0. 5-500 ng/ml) in a dose-dependent manner. MIP-1alpha and TNFalpha were 
      detected in culture supernatants of LPS-stimulated samples with concentrations 
      about 8 times as high as those of samples cultured with no such stimulus. The 
      addition of IL-1beta resulted in an increase in the release of IL-6 and MCP-1, 
      similar to that observed with LPS stimulation, but failed to increase the 
      production of TNFalpha. MIP-1alpha production was only marginally enhanced by 
      IL-1beta. In conclusion, our experimental system is suitable for the 
      investigation of chemokine and cytokine production by the human peritoneum, with 
      the aim of assessing aspects of local immunocompetence.
FAU - Riese, J
AU  - Riese J
AD  - Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany. 
      Jutta.Riese@chir.med.uni-erlangen.de
FAU - Denzel, C
AU  - Denzel C
FAU - Zowe, M
AU  - Zowe M
FAU - Mehler, C
AU  - Mehler C
FAU - Hohenberger, W
AU  - Hohenberger W
FAU - Haupt, W
AU  - Haupt W
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Eur Surg Res
JT  - European surgical research. Europaische chirurgische Forschung. Recherches 
      chirurgicales europeennes
JID - 0174752
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Survival
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Culture Techniques
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Inflammatory Proteins/*metabolism
MH  - Peritoneum/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - 8704 [pii]
AID - 10.1159/000008704 [doi]
PST - ppublish
SO  - Eur Surg Res. 1999;31(3):281-8. doi: 10.1159/000008704.