PMID- 10352939
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20061115
IS  - 1018-4813 (Print)
IS  - 1018-4813 (Linking)
VI  - 7
IP  - 4
DP  - 1999 May-Jun
TI  - Detailed transcript map of a 810-kb region at 11p14 involving identification of 
      10 novel human 3' exons.
PG  - 487-95
AB  - A limited number of genes, including the human brain-derived neutrotrophic factor 
      (BDNF) gene, have been identified in the human chromosome 11p14 region. Since 
      this area is involved in a genetic disorder (WAGR syndrome) and because of 
      interest in studying the regulation of the human BDNF gene, we have established a 
      detailed transcript map of a 810-kb region clone in a yeast artificial chromosome 
      (YAC), corresponding to a portion of this genomic locus. A set of nested deletion 
      mutants has been generated to map genes at a mean resolution of 75kb. Four genic 
      markers from available mapping databases have been mapped on the YAC. Ten 
      potential novel human exons have been isolated by a 3' terminal exon trapping 
      procedure directly applied to purified YAC DNA. Most of these exons display 
      polyadenylation signals and they all yield positive signals in RT-PCR 
      experiments, confirming their status of transcribed sequences. The BDNF gene is 
      now co-localised with three other genes on a 120 kb DNA fragment.
FAU - Guillemot, F
AU  - Guillemot F
AD  - Genetique Moleculaire et Biologie du Developpement, CNRS-UPR420, Villejuif, 
      France. guillemo@infobiogen.fr
FAU - Auffray, C
AU  - Auffray C
FAU - Devignes, M D
AU  - Devignes MD
LA  - eng
SI  - GENBANK/AJ011593
SI  - GENBANK/AJ011594
SI  - GENBANK/AJ011595
SI  - GENBANK/AJ011596
SI  - GENBANK/AJ011597
SI  - GENBANK/AJ011598
SI  - GENBANK/AJ011599
SI  - GENBANK/AJ011600
SI  - GENBANK/AJ011601
SI  - GENBANK/AJ011602
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Hum Genet
JT  - European journal of human genetics : EJHG
JID - 9302235
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Exons/*genetics
MH  - Humans
MH  - Microsatellite Repeats/genetics
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - *Transcription, Genetic
MH  - WAGR Syndrome/genetics
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - 10.1038/sj.ejhg.5200321 [doi]
PST - ppublish
SO  - Eur J Hum Genet. 1999 May-Jun;7(4):487-95. doi: 10.1038/sj.ejhg.5200321.