PMID- 10353460
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20220317
IS  - 1434-6621 (Print)
IS  - 1434-6621 (Linking)
VI  - 37
IP  - 3
DP  - 1999 Mar
TI  - Association between reduced human leukocyte antigen (HLA)-DR expression on blood 
      monocytes and increased plasma level of interleukin-10 in patients with severe 
      burns.
PG  - 193-8
AB  - Severe thermal injury causes an immune dysfunction which includes a decrease of 
      monocyte human leukocyte antigen DR (HLA-DR) expression. Interleukin-10 exerts a 
      negative influence on this parameter in vitro. In this study we determined the 
      prognostic value of reduced monocyte HLA-DR expression with regard to infectious 
      complications, and the in vivo association between monocyte HLA-DR and plasma 
      interleukin-10 concentration. Both quantities were measured serially in 19 
      patients with severe burns. HLA-DR expression was determined by direct 
      immunofluorescence on a flow cytometer, and interleukin-10 was measured by ELISA. 
      After burn trauma the percentage of HLA-DR expressing monocytes fell markedly 
      (median: 53% at day 2, 36% at day 4, 31% at day 7, 28% at day 9, 35% at day 12, 
      and 42% at day 14; compared to 93% for healthy volunteers). Moreover, patients 
      who became septic showed lower monocyte HLA-DR expression than non-septic 
      patients; the differences were significant at day 2 (p < 0.01) and day 7 (p < 
      0.05). Plasma concentrations of interleukin-10 increased after thermal injury 
      (median: 40 ng/l at day 2, 43 ng/l at day 4, 77 ng/l at day 7, 120 ng/l at day 9, 
      63 ng/l at day 12, and 82 ng/l at day 14). Individual HLA-DR expression and 
      interleukin-10 concentration were negatively correlated, the association reaching 
      statistical significance at day 4 (p=0.006) and day 7 (p=0.031). Thus, after 
      severe burn injury monocyte HLA-DR expression has prognostic value and is 
      negatively associated with interleukin-10 plasma concentration.
FAU - Sachse, C
AU  - Sachse C
AD  - Institut fur Klinische Chemie II, Medizinische Hochschule Hannover, Germany.
FAU - Prigge, M
AU  - Prigge M
FAU - Cramer, G
AU  - Cramer G
FAU - Pallua, N
AU  - Pallua N
FAU - Henkel, E
AU  - Henkel E
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (HLA-DR Antigens)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Burns/blood/complications/*immunology
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HLA-DR Antigens/*blood
MH  - Humans
MH  - Interleukin-10/*blood
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology
MH  - Sepsis/complications/microbiology
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - 10.1515/CCLM.1999.036 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 1999 Mar;37(3):193-8. doi: 10.1515/CCLM.1999.036.