PMID- 10353843
OWN - NLM
STAT- MEDLINE
DCOM- 19990623
LR  - 20181201
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 22
DP  - 1999 Jun 1
TI  - Introduction of mannose binding protein-type phosphatidylinositol recognition 
      into pulmonary surfactant protein A.
PG  - 7321-31
AB  - Pulmonary surfactant protein A (SP-A) and mannose-binding protein A (MBP-A) are 
      collectins in the C-type lectin superfamily. These collectins exhibit unique 
      lipid binding properties. SP-A binds to dipalmitoyl phosphatidylcholine (DPPC) 
      and galactosylceramide (GalCer) and MBP-A binds to phosphatidylinositol (PI). 
      SP-A also interacts with alveolar type II cells. Monoclonal antibodies (mAbs PE10 
      and PC6) that recognize human SP-A inhibit the interactions of SP-A with lipids 
      and alveolar type II cells. We mapped the epitopes for anti-human SP-A mAbs by a 
      phage display peptide library. Phage selected by mAbs displayed the consensus 
      peptide sequences that are nearly identical to 184TPVNYTNWYRG194 of human SP-A. 
      The synthetic peptide GTPVNYTNWYRG completely blocked the binding of mAbs to 
      human SP-A. Chimeric proteins were generated in which the rat SP-A region 
      Thr174-Gly194 or the human SP-A region Ser174-Gly194 was replaced with the MBP-A 
      region Thr164-Asp184 (rat ama4 or hu ama4, respectively). The mAbs failed to bind 
      hu ama4. Rat ama4 bound to an affinity matrix on mannose-sepharose but lost all 
      of the SP-A functions except carbohydrate binding and Ca2+-independent GalCer 
      binding. Strikingly, the rat ama4 chimera acquired the PI binding property that 
      MBP-A exhibits. This study demonstrates that the amino acid residues 174-194 of 
      SP-A and the corresponding region of MBP-A are critical for SP-A-type II cell 
      interaction and Ca2+-dependent lipid binding of collectins.
FAU - Chiba, H
AU  - Chiba H
AD  - Department of Biochemistry, Sapporo Medical University School of Medicine, 
      South-1 West-17, Chuo-ku, Sapporo 060-8556, Japan.
FAU - Sano, H
AU  - Sano H
FAU - Saitoh, M
AU  - Saitoh M
FAU - Sohma, H
AU  - Sohma H
FAU - Voelker, D R
AU  - Voelker DR
FAU - Akino, T
AU  - Akino T
FAU - Kuroki, Y
AU  - Kuroki Y
LA  - eng
GR  - HL45286/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carrier Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Proteolipids)
RN  - 0 (Pulmonary Surfactant-Associated Protein A)
RN  - 0 (Pulmonary Surfactant-Associated Proteins)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (Recombinant Fusion Proteins)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Binding Sites, Antibody/drug effects
MH  - Binding, Competitive
MH  - Carrier Proteins/genetics/*metabolism
MH  - Epitopes/immunology
MH  - Humans
MH  - Male
MH  - Mannose/*metabolism
MH  - Mannose-Binding Lectins
MH  - Phosphatidylinositols/antagonists & inhibitors/*metabolism
MH  - Proteolipids/antagonists & inhibitors/genetics/immunology/*metabolism
MH  - Pulmonary Alveoli/cytology/drug effects/metabolism
MH  - Pulmonary Surfactant-Associated Protein A
MH  - Pulmonary Surfactant-Associated Proteins
MH  - Pulmonary Surfactants/antagonists & inhibitors/genetics/immunology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Fusion Proteins/genetics/metabolism/pharmacology
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - bi990353e [pii]
AID - 10.1021/bi990353e [doi]
PST - ppublish
SO  - Biochemistry. 1999 Jun 1;38(22):7321-31. doi: 10.1021/bi990353e.