PMID- 10357410
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20210409
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 85
IP  - 11
DP  - 1999 Jun 1
TI  - Can immunohistochemical markers and mitotic rate improve prognostic precision in 
      patients with primary melanoma?
PG  - 2391-9
AB  - BACKGROUND: In addition to tumor thickness, several other prognostic parameters 
      have been identified in primary human melanomas. Some are available readily 
      (localization, gender, age, and ulceration). Others must be evaluated with a 
      moderate or even substantial amount of work (mitoses and immunohistochemical 
      markers). This study was undertaken to determine whether this extra effort is 
      justified because it actually improves the precision of prognostic statements. 
      METHODS: Immunohistologic markers were determined on frozen sections from 691 
      biopsies of human melanomas with the immunoperoxidase method. Univariate and 
      multivariate Cox regression analyses were performed with metastases and with 
      death as endpoints. RESULTS: Fifteen parameters were related to disease free 
      survival in univariate Cox regression analysis: tumor thickness, ulceration, 
      localization, gender, age, mitoses, and the immunohistochemical markers very late 
      antigen (VLA)-2, human leukocyte antigen (HLA)-ABC, HLA-DR, NKI-beteb, Mel 14, 
      intercellular adhesion molecule (ICAM-1), K-1-2, G-7-E2, and H-2-4-7. Three of 
      the easily available parameters exhibited independent significance in 
      multivariate Cox regression analysis: tumor thickness, ulceration, and 
      localization. If mitotic rate was included in this model, then it had independent 
      prognostic significance but ulceration was no longer significant. However, the 
      model that included tumor thickness, localization, and ulceration had a slightly 
      higher overall chi-square test score, indicating a better performance compared 
      with thickness, localization, and mitoses. The model that included tumor 
      thickness, localization, and mitoses could not be improved by any of the 
      immunohistochemical markers in this study. CONCLUSIONS: Nine immunohistochemical 
      markers with established prognostic significance for primary human melanoma were 
      not found to improve a prognostic model that included tumor thickness, 
      localization, and mitoses. If mitoses was replaced by ulceration, then the model 
      performed slightly better, although ulceration was not significant in the 
      presence of mitoses.
FAU - Ostmeier, H
AU  - Ostmeier H
AD  - Fachklinik Hornheide, Munster, Germany.
FAU - Fuchs, B
AU  - Fuchs B
FAU - Otto, F
AU  - Otto F
FAU - Mawick, R
AU  - Mawick R
FAU - Lippold, A
AU  - Lippold A
FAU - Krieg, V
AU  - Krieg V
FAU - Suter, L
AU  - Suter L
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Melanoma/*chemistry
MH  - Mitosis/*physiology
MH  - Multivariate Analysis
MH  - Prognosis
MH  - Reproducibility of Results
EDAT- 1999/06/05 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/06/05 10:00
PHST- 1999/06/05 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/06/05 10:00 [entrez]
AID - 10.1002/(SICI)1097-0142(19990601)85:11<2391::AID-CNCR14>3.0.CO;2-I [pii]
AID - 10.1002/(sici)1097-0142(19990601)85:11<2391::aid-cncr14>3.0.co;2-i [doi]
PST - ppublish
SO  - Cancer. 1999 Jun 1;85(11):2391-9. doi: 
      10.1002/(sici)1097-0142(19990601)85:11<2391::aid-cncr14>3.0.co;2-i.