PMID- 10357825
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 11
DP  - 1999 Jun 1
TI  - Post-termination ribosome interactions with the 5'UTR modulate yeast mRNA 
      stability.
PG  - 3139-52
AB  - A novel form of post-transcriptional control is described. The 5' untranslated 
      region (5'UTR) of the Saccharomyces cerevisiae gene encoding the AP1-like 
      transcription factor Yap2 contains two upstream open reading frames (uORF1 and 
      uORF2). The YAP2-type of uORF functions as a cis-acting element that attenuates 
      gene expression at the level of mRNA turnover via termination-dependent decay. 
      Release of post-termination ribosomes from the YAP2 5'UTR causes accelerated 
      decay which is largely independent of the termination modulator gene UPF1. Both 
      of the YAP2 uORFs contribute to the destabilization effect. A G/C-rich stop codon 
      context, which seems to promote ribosome release, allows an uORF to act as a 
      transferable 5'UTR-destabilizing element. Moreover, termination-dependent 
      destabilization is potentiated by stable secondary structure 3' of the uORF stop 
      codon. The potentiation of uORF-mediated destabilization is eliminated if the 
      secondary structure is located further downstream of the uORF, and is also 
      influenced by a modulatory mechanism involving eIF2. Destabilization is therefore 
      linked to the kinetics of acquisition of reinitiation-competence by 
      post-termination ribosomes in the 5'UTR. Our data explain the destabilizing 
      properties of YAP2-type uORFs and also support a more general model for the mode 
      of action of other known uORFs, such as those in the GCN4 mRNA.
FAU - Vilela, C
AU  - Vilela C
AD  - Post-transcriptional Control Group, Department of Biomolecular Sciences, 
      University of Manchester Institute of Science and Technology (UMIST), PO Box 88, 
      Manchester M60 1QD, UK.
FAU - Ramirez, C V
AU  - Ramirez CV
FAU - Linz, B
AU  - Linz B
FAU - Rodrigues-Pousada, C
AU  - Rodrigues-Pousada C
FAU - McCarthy, J E
AU  - McCarthy JE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (5' Untranslated Regions)
RN  - 0 (CAD1 protein, S cerevisiae)
RN  - 0 (Codon, Initiator)
RN  - 0 (Codon, Terminator)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (Fungal Proteins)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP1 protein, S cerevisiae)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 3.6.1.- (NAM7 protein, S cerevisiae)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - 5' Untranslated Regions/chemistry/genetics/*metabolism
MH  - Base Sequence
MH  - Codon, Initiator/genetics
MH  - Codon, Terminator/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Eukaryotic Initiation Factor-2/genetics/physiology
MH  - Fungal Proteins/genetics
MH  - Gene Expression Regulation, Fungal
MH  - Genes, Fungal/genetics/physiology
MH  - Half-Life
MH  - Models, Genetic
MH  - Mutation
MH  - Nucleic Acid Conformation
MH  - Open Reading Frames/genetics
MH  - Protein Biosynthesis/genetics
MH  - Protein Kinases/genetics
MH  - RNA Helicases/genetics/metabolism
MH  - RNA, Fungal/chemistry/genetics/metabolism
MH  - RNA, Messenger/chemistry/genetics/*metabolism
MH  - Ribosomes/*metabolism/physiology
MH  - Saccharomyces cerevisiae/*genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Transcription Factors/genetics
PMC - PMC1171395
EDAT- 1999/06/05 00:00
MHDA- 1999/06/05 00:01
PMCR- 2000/06/01
CRDT- 1999/06/05 00:00
PHST- 1999/06/05 00:00 [pubmed]
PHST- 1999/06/05 00:01 [medline]
PHST- 1999/06/05 00:00 [entrez]
PHST- 2000/06/01 00:00 [pmc-release]
AID - 10.1093/emboj/18.11.3139 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jun 1;18(11):3139-52. doi: 10.1093/emboj/18.11.3139.