PMID- 10357839
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 40
IP  - 6
DP  - 1999 Jun
TI  - Atypical expression of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in 
      subcutaneous adipose tissue of male rats.
PG  - 1071-7
AB  - The mRNAs encoding mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 
      (mtHMG-CoA synthase), the rate limiting enzyme in ketone body production, are 
      highly expressed in subcutaneous (SC) and, to a lesser extent, in peri-epididymal 
      (PE) rat adipose tissues. This atypical mtHMG-CoA synthase gene expression is 
      dependent on the age (from 9 weeks of age) and sex (higher in male than in 
      female) of the rats. In contrast, the expression of mtHMG-CoA synthase in SC 
      adipose deposit is independent of the nutritional state (fed versus starved) or 
      of the thermic environment (24 degrees C versus 4 degrees C). The expression of 
      mtHMG-CoA synthase is suppressed in SC fat pads of castrated male rats whereas 
      treatment of castrated rats with testosterone restores a normal level of 
      expression. Moreover, testosterone injection induces the expression mtHMG-CoA 
      synthase in SC adipose tissue of age-matched females. The presence of the 
      mtHMG-CoA synthase immunoreactive protein confers to mitochondria isolated from 
      SC adipose deposits, the capacity to produce ketone bodies at a rate similar to 
      that found in liver mitochondria (SC = 13.7 +/- 0.7, liver = 16.4 +/- 1.4 
      nmol/min/mg prot). mtHMG-CoA synthase is expressed in the stromal vascular 
      fraction (SVF) whatever the adipose deposit considered. While acetyl-CoA 
      carboxylase (ACC) is only expressed in mature adipocytes, the other lipogenic 
      enzymes, fatty acid synthase (FAS) and citrate cleavage enzyme (CCE), are 
      expressed both in SVF cells and mature adipocytes. The expression of lipogenic 
      enzyme genes is markedly reduced in adipocytes but not in SVF cells isolated from 
      48-h starved male rats. When SVF is subfractionated, mtHMG-CoA synthase mRNAs are 
      mainly recovered in two fractions containing poorly digested structures such as 
      microcapillaries whereas the lowest expression is found in the pre-adipocyte 
      fraction. Interestingly, FAS and CCE mRNAs co-segregate with mtHMG-CoA synthase 
      mRNA. The possible physiological relevance of such atypical expression of 
      mtHMG-CoA synthase is discussed.
FAU - Thumelin, S
AU  - Thumelin S
AD  - Endocrinologie Metabolisme et Developpement, UPR 1524 CNRS, 9, rue Jules Hetzel, 
      92190 Meudon, France.
FAU - Kohl, C
AU  - Kohl C
FAU - Girard, J
AU  - Girard J
FAU - Pegorier, J P
AU  - Pegorier JP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Ketone Bodies)
RN  - 0 (RNA, Messenger)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 2.3.3.10 (Hydroxymethylglutaryl-CoA Synthase)
SB  - IM
MH  - Adipose Tissue/*enzymology/ultrastructure
MH  - Aging
MH  - Animals
MH  - Female
MH  - *Gene Expression Regulation, Enzymologic/drug effects
MH  - Hydroxymethylglutaryl-CoA Synthase/*genetics
MH  - Ketone Bodies/biosynthesis
MH  - Male
MH  - Mitochondria/*enzymology
MH  - Orchiectomy
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Sex Characteristics
MH  - Starvation/enzymology
MH  - Testosterone/pharmacology
EDAT- 1999/06/05 00:00
MHDA- 1999/06/05 00:01
CRDT- 1999/06/05 00:00
PHST- 1999/06/05 00:00 [pubmed]
PHST- 1999/06/05 00:01 [medline]
PHST- 1999/06/05 00:00 [entrez]
AID - S0022-2275(20)33511-2 [pii]
PST - ppublish
SO  - J Lipid Res. 1999 Jun;40(6):1071-7.