PMID- 10359336
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20081121
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 7
DP  - 1999 Jun
TI  - Effects of the neurotrophin brain-derived neurotrophic factor in an experimental 
      model of retinal detachment.
PG  - 1530-44
AB  - PURPOSE: To examine the effects of brain-derived neurotrophic factor (BDNF) in an 
      animal model of retinal detachment. METHODS: Cat retinas were detached from the 
      retinal pigment epithelium for either 7 or 28 days. Animals received either an 
      intravitreal injection of BDNF (100 ILg) or phosphate-buffered saline (PBS), the 
      vehicle for BDNF. Retinas were evaluated using morphology and 
      immunocytochemistry. The width of the outer segment zone was measured, and the 
      retinas were evaluated for changes in protein expression by labeling with 
      antibodies to rod opsin, phosducin, synaptophysin, calbindin D, and glial 
      fibrillary acidic protein (GFAP). The effect of BDNF on both proliferation and 
      apoptotic cell death was examined. RESULTS: Although there was variability in the 
      treated retinas, most of the animals receiving BDNF had well-organized outer 
      segments that were longer than those in vehicle-treated controls. 
      Immunocytochemistry revealed that treated retinas had consistently less opsin 
      redistribution to the plasma membrane, less phosducin upregulation, and fewer 
      calbindin D-labeled horizontal cell processes. BDNF did not reduce overall cell 
      death in the detachments or death of photoreceptors by apoptosis. However, it 
      significantly reduced the proliferative response of Miller cells and the extent 
      of upregulation of GFAP. CONCLUSIONS. The results suggest that BDNF may aid in 
      the recovery of the retina after reattachment by maintaining the surviving 
      photoreceptor cells, by reducing the gliotic effects in Muller cells, and perhaps 
      by promoting outer segment regeneration.
FAU - Lewis, G P
AU  - Lewis GP
AD  - Neuroscience Research Institute, University of California, Santa Barbara 93106, 
      USA.
FAU - Linberg, K A
AU  - Linberg KA
FAU - Geller, S F
AU  - Geller SF
FAU - Guerin, C J
AU  - Guerin CJ
FAU - Fisher, S K
AU  - Fisher SK
LA  - eng
GR  - EY00888/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Eye Proteins)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cats
MH  - Cell Count
MH  - Cell Death/drug effects
MH  - Cell Division
MH  - *Disease Models, Animal
MH  - Eye Proteins/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - In Situ Nick-End Labeling
MH  - Injections
MH  - Ki-67 Antigen/metabolism
MH  - Microscopy, Confocal
MH  - Photoreceptor Cells, Vertebrate/*drug effects/metabolism/ultrastructure
MH  - Retinal Degeneration/etiology/metabolism/pathology/*prevention & control
MH  - Retinal Detachment/*complications/metabolism/pathology
MH  - Vitreous Body
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Jun;40(7):1530-44.