PMID- 10360812
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20221207
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 82
IP  - 1
DP  - 1999 Jul 2
TI  - Monocyte-chemo-attractant-protein-1 (MCP-1)-gene expression in cervical 
      intra-epithelial neoplasias and cervical carcinomas.
PG  - 6-11
AB  - Chemokines play a central role in the chemotactic activation of immunological 
      effector cells. One of the currently best characterized chemokines is the 
      monocyte-chemo-attractant protein-1 (MCP-1), which is involved in the cross-talk 
      with cells of the monocyte-macrophage lineage. Since macrophages and 
      macrophage-derived cytokines appear to be important in the transcriptional 
      regulation of "high-risk" types of human papillomaviruses (HPV), we monitored 
      MCP-1 expression by in situ hybridization (ISH) in histologically distinct stages 
      of cervical intra-epithelial neoplasms (CIN), cervical cancer and 
      non-HPV-associated cases of erosive endocervicitis. Here, we demonstrate that 
      high-grade dysplasia (CIN III, n = 9) completely lacks both MCP-1 expression and 
      CD68+-macrophage infiltration, while MCP-1-specific signals were occasionally 
      detectable in one out of 5 CIN-II and in one out of 3 CIN-I lesions. Inspection 
      of hyperplastic squamous epithelium adjacent to cervical carcinomas reveals high 
      MCP-1 expression and accumulation of infiltrating macrophages. In contrast, no 
      macrophages could be detected in corresponding hyperplastic tissue areas 
      surrounding CIN-II and CIN-III lesions, although MCP-1 was found to be highly 
      expressed. Finally, in agreement with our earlier in vitro data, invasive 
      carcinomas of the cervix uteri showed MCP-1-specific hybridization signals and 
      macrophage infiltration only in the stroma surrounding the carcinoma cells and in 
      endothelial cells of capillaries, especially at the invasion front of the tumor, 
      while the inner mass of the carcinomas was completely negative. On the other 
      hand, ISH and histochemical evaluation of inflammatory, non-HPV-associated cases 
      of erosive endocervicitis indicate strong MCP-1 expression, which is regularly 
      accompanied by chemotactic appearance of macrophages. These observations indicate 
      that dysregulation of MCP-1-gene expression may represent an important step 
      during HPV-linked carcinogenesis, allowing the escape of virus-positive cells 
      from local immune response.
FAU - Kleine-Lowinski, K
AU  - Kleine-Lowinski K
AD  - Friedrich Schiller Universitat, Klinik fur Gynakologie und Institut fur 
      Pathologie, Jena, Germany.
FAU - Gillitzer, R
AU  - Gillitzer R
FAU - Kuhne-Heid, R
AU  - Kuhne-Heid R
FAU - Rosl, F
AU  - Rosl F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Chemokine CCL2/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Macrophages/pathology
MH  - Middle Aged
MH  - Papillomaviridae
MH  - RNA, Messenger/*analysis
MH  - Tumor Virus Infections/complications/immunology
MH  - Uterine Cervical Neoplasms/etiology/*immunology/pathology
MH  - Uterine Cervical Dysplasia/etiology/*immunology/pathology
EDAT- 1999/06/09 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/06/09 10:00
PHST- 1999/06/09 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/06/09 10:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19990702)82:1<6::AID-IJC2>3.0.CO;2-3 [pii]
AID - 10.1002/(sici)1097-0215(19990702)82:1<6::aid-ijc2>3.0.co;2-3 [doi]
PST - ppublish
SO  - Int J Cancer. 1999 Jul 2;82(1):6-11. doi: 
      10.1002/(sici)1097-0215(19990702)82:1<6::aid-ijc2>3.0.co;2-3.