PMID- 10361131
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 93
IP  - 12
DP  - 1999 Jun 15
TI  - Activated dendritic cells from bone marrow cells of mice receiving 
      cytokine-expressing tumor cells are associated with the enhanced survival of mice 
      bearing syngeneic tumors.
PG  - 4328-35
AB  - Dendritic cells (DCs), which phagocytose antigens and subsequently proliferate 
      and migrate, may be the most powerful antigen-presenting cells that activate 
      naive T cells. To determine their role in the immune response to tumors, we used 
      WEHI-3B murine leukemia cells transduced with adenovirus vectors expressing 
      cytokines. We found that mixtures of irradiated cells expressing 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) plus those expressing 
      interleukin-4 (IL-4) or tumor necrosis factor alpha (TNFalpha) protected mice 
      against WEHI-3B-induced leukemias. When bone marrow mononuclear cells (BMMNCs) 
      obtained from mice that had been injected with irradiated, cytokine-expressing 
      tumor cells were injected into tumor-bearing mice, the survival of the latter was 
      significantly prolonged; the longest survival was observed in mice receiving 
      BMMNCs containing an increased number of DCs from animals injected with a mixture 
      of tumor cells expressing GM-CSF with those expressing IL-4. Assay for 
      antileukemic effects in spleen of the latter animals showed specific antitumor 
      cytotoxicity against WEHI-3B, suggesting that DCs from donor mice activate 
      specific T cells in the tumor-bearing recipients. These results suggest that the 
      infusion of syngeneic BMMNCs stimulated with cytokine-expressing tumor cells may 
      be effective in treating certain types of tumors.
FAU - Fujii, S
AU  - Fujii S
AD  - Center for Bone Marrow Transplantation and Immunotherapy, Institute for Clinical 
      Research, Kumamoto National Hospital, Kumamoto, Japan.
FAU - Hamada, H
AU  - Hamada H
FAU - Fujimoto, K
AU  - Fujimoto K
FAU - Shimomura, T
AU  - Shimomura T
FAU - Kawakita, M
AU  - Kawakita M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Bone Marrow Cells/*immunology
MH  - Cancer Vaccines
MH  - Cricetinae
MH  - Cytokines/*genetics/*immunology
MH  - Dendritic Cells/*immunology
MH  - *Gene Expression
MH  - Genetic Vectors
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/immunology
MH  - *Immunotherapy
MH  - Interleukin-4/genetics/immunology
MH  - Leukemia, Experimental/immunology/*therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - S0006-4971(20)59463-9 [pii]
PST - ppublish
SO  - Blood. 1999 Jun 15;93(12):4328-35.