PMID- 10362789
OWN - NLM
STAT- MEDLINE
DCOM- 19990714
LR  - 20240414
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 154
IP  - 6
DP  - 1999 Jun
TI  - Stable expression in Chinese hamster ovary cells of mutated tau genes causing 
      frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17).
PG  - 1649-56
AB  - Extensive neuronal loss and aggregation of tau as cytoplasmic inclusions in 
      neurons and glial cells in selected cortical and subcortical regions is the most 
      striking characteristic of frontotemporal dementia and parkinsonism linked to 
      chromosome 17, which is caused by exonic or intronic mutations in the tau gene. 
      Here, we examined the effects of four exonic mutations in four-repeat tau using 
      stably transfected Chinese hamster ovary cells. The proportion of polymerized 
      tubulin was the largest in the P301L transfectant. G272V and P301L transfectants 
      showed greater instability of microtubules in the presence of Colcemid than 
      wild-type tau, V337M, or R406W transfectants. Thus no distinct phenotypes were 
      shared by the mutant tau transfectants with regard to microtubule assembly and 
      stability. Unexpectedly, R406W showed low and negligible levels of 
      phosphorylation at Thr 231 and Ser 396, respectively, in the transfectant. This 
      presents a sharp contrast to the observation that tau aggregates in 
      R406W-affected brains are heavily phosphorylated at these two sites. This result 
      suggests that hyperphosphorylation at these sites cannot occur in the tau R406W 
      bound to microtubules, and thus that the hyperphosphorylated species of tau may 
      be generated only after disruption of microtubules.
FAU - Matsumura, N
AU  - Matsumura N
AD  - Department of Neuropathology, Faculty of Medicine, University of Tokyo, Japan.
FAU - Yamazaki, T
AU  - Yamazaki T
FAU - Ihara, Y
AU  - Ihara Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (tau Proteins)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - CHO Cells
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Cricetinae
MH  - Cytochalasin B/pharmacology
MH  - Demecolcine/pharmacology
MH  - Dementia/*genetics
MH  - Gene Expression
MH  - Genetic Linkage
MH  - Humans
MH  - Immunohistochemistry
MH  - Microtubules/drug effects/physiology
MH  - *Mutation
MH  - Parkinson Disease/*genetics
MH  - Subcellular Fractions/metabolism
MH  - Transfection
MH  - tau Proteins/*biosynthesis/*genetics/metabolism
PMC - PMC1866642
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
PMCR- 1999/12/01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
PHST- 1999/12/01 00:00 [pmc-release]
AID - S0002-9440(10)65420-X [pii]
AID - 1748 [pii]
AID - 10.1016/S0002-9440(10)65420-X [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Jun;154(6):1649-56. doi: 10.1016/S0002-9440(10)65420-X.