PMID- 10366558
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 258
IP  - 2
DP  - 1999 Jun 5
TI  - Specific and nonspecific immune stimulation of MHC-II-deficient mice results in 
      chronic HSV-1 infection of the trigeminal ganglia following ocular challenge.
PG  - 208-16
AB  - Ocular herpes simplex virus type 1 (HSV-1) infection of MHC-II-deficient mice 
      (AO/Obeta mice) or their parental C57BL/6J wild-type mice resulted in the 
      establishment of typical HSV-1 latent infections in the trigeminal ganglia (TG) 
      of the surviving mice by day 28 postinfection. Latency was characterized by the 
      complete absence of infectious virus in TG extracts, the ability to recover 
      latent virus only following prolonged tissue culture cultivation of explanted TG, 
      and the presence of HSV-1 DNA in TG extracts. When mice were vaccinated prior to 
      ocular HSV-1 challenge, latency appeared unaltered in the C57BL/6J wild-type 
      mice. However, in AO/Obeta mice, clearance of virus from the TG appeared to be 
      seriously impaired, resulting in a chronic productive infection, rather than a 
      latent infection. Infectious virus was readily detected in TG extracts of 
      vaccinated AO/Obeta mice until at least 63 days postinfection. Glycoprotein B 
      mRNA was also readily detected, confirming continued viral transcription. These 
      chronic infections occurred regardless of whether the AO/Obeta mice were 
      vaccinated with HSV-1-specific antigens (i.e., live HSV-1 strain KOS, 
      recombinantly expressed HSV-1 glycoprotein D plus Freund's adjuvant, or a mixture 
      of seven recombinantly expressed HSV-1 glycoproteins plus adjuvant) or 
      non-HSV-1-specific antigens (i.e., tissue culture medium plus 5% fetal bovine 
      serum, the expression vector plus adjuvant, or adjuvant alone). Passive transfer 
      of HSV-1 neutralizing antibody to vaccinated AO/Obeta mice between days 0 and 28 
      post-ocular challenge did not clear infectious virus from the TG. Passive 
      transfer of anti-HSV-1 antibody or purified naive mouse serum to unvaccinated 
      AO/Obeta mice on days 3 or 6 post-HSV-1 ocular challenge also resulted in 
      chronic, rather than latent, infection of the TG. Passive transfer of naive sera 
      from B-cell-deficient mice or injection of keyhole limpet hemocyanin or purified 
      IgG, but not PBS or dextran, 3 days after HSV-1 challenge also resulted in 
      chronic infection of the TG.
CI  - Copyright 1999 Academic Press.
FAU - Ghiasi, H
AU  - Ghiasi H
AD  - Ophthalmology Research, Cedars-Sinai Burn and Allen Research Institute, 
      CSMC-Davis Building, Room 5072, 8700 Beverly Boulevard, Los Angeles, California, 
      90048, USA. ghiasi@CSMC.Edu
FAU - Perng, G C
AU  - Perng GC
FAU - Hofman, F M
AU  - Hofman FM
FAU - Cai, S
AU  - Cai S
FAU - Nesburn, A B
AU  - Nesburn AB
FAU - Wechsler, S L
AU  - Wechsler SL
LA  - eng
GR  - EY09224/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Viral Vaccines)
SB  - IM
EIN - Virology 1999 Sep 1;261(2):367. Pemg GC [corrected to Perng GC]
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Cattle
MH  - Cell-Free System
MH  - Chronic Disease
MH  - Disease Models, Animal
MH  - Eye/immunology/virology
MH  - Herpes Simplex/*immunology
MH  - Herpesvirus 1, Human/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Immunization, Passive
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rabbits
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Trigeminal Ganglion/immunology/*virology
MH  - Vaccination
MH  - Viral Vaccines/immunology
MH  - Virus Latency/*immunology
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0042682299997106 [pii]
AID - 10.1006/viro.1999.9710 [doi]
PST - ppublish
SO  - Virology. 1999 Jun 5;258(2):208-16. doi: 10.1006/viro.1999.9710.