PMID- 10366587
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20240326
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 6
DP  - 1999 Jun 14
TI  - Intron-independent association of splicing factors with active genes.
PG  - 1133-43
AB  - The cell nucleus is organized as discrete domains, often associated with specific 
      events involved in chromosome organization, replication, and gene expression. We 
      have examined the spatial and functional relationship between the sites of heat 
      shock gene transcription and the speckles enriched in splicing factors in primary 
      human fibroblasts by combining immunofluorescence and fluorescence in situ 
      hybridization (FISH). The hsp90alpha and hsp70 genes are inducibly regulated by 
      exposure to stress from a low basal level to a high rate of transcription; 
      additionally the hsp90alpha gene contains 10 introns whereas the hsp70 gene is 
      intronless. At 37 degrees C, only 30% of hsp90alpha transcription sites are 
      associated with speckles whereas little association is detected with the hsp70 
      gene, whose constitutive expression is undetectable relative to the hsp90alpha 
      gene. Upon exposure of cells to heat shock, the heavy metal cadmium, or the amino 
      acid analogue azetidine, transcription at the hsp90alpha and hsp70 gene loci is 
      strongly induced, and both hsp transcription sites become associated with 
      speckles in >90% of the cells. These results reveal a clear disconnection between 
      the presence of intervening sequences at specific gene loci and the association 
      with splicing factor-rich regions and suggest that subnuclear structures 
      containing splicing factors are associated with sites of transcription.
FAU - Jolly, C
AU  - Jolly C
AD  - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern 
      University, Evanston, Illinois 60208, USA. c-jolly@nwu.edu
FAU - Vourc'h, C
AU  - Vourc'h C
FAU - Robert-Nicoud, M
AU  - Robert-Nicoud M
FAU - Morimoto, R I
AU  - Morimoto RI
LA  - eng
GR  - R01 GM038109/GM/NIGMS NIH HHS/United States
GR  - R37 GM038109/GM/NIGMS NIH HHS/United States
GR  - GM38109/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Azetidines)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 00BH33GNGH (Cadmium)
RN  - 147153-65-9 (SRSF2 protein, human)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
RN  - 37S883XDWR (azetidine)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Azetidines/pharmacology
MH  - Cadmium/pharmacology
MH  - Cells, Cultured
MH  - Fibroblasts
MH  - Fluorescent Antibody Technique
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - HSP90 Heat-Shock Proteins/*genetics
MH  - Heat-Shock Response/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns/*genetics
MH  - Nuclear Proteins/*metabolism
MH  - RNA Polymerase II/metabolism
MH  - RNA Splicing/drug effects/genetics
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - *Ribonucleoproteins
MH  - Ribonucleoproteins, Small Nuclear/metabolism
MH  - Serine-Arginine Splicing Factors
MH  - Spliceosomes/drug effects/genetics/*metabolism
MH  - Temperature
MH  - Transcription, Genetic/drug effects/*genetics
MH  - Transcriptional Activation
PMC - PMC2133154
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
PMCR- 1999/12/14
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
PHST- 1999/12/14 00:00 [pmc-release]
AID - 10.1083/jcb.145.6.1133 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Jun 14;145(6):1133-43. doi: 10.1083/jcb.145.6.1133.