PMID- 10366699
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 115
IP  - 1
DP  - 1999 Jun 8
TI  - Synergistic effects of brain-derived neurotrophic factor and ciliary neurotrophic 
      factor on cultured basal forebrain cholinergic neurons from postnatal 2-week-old 
      rats.
PG  - 25-32
AB  - Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, 
      and ciliary neurotrophic factor (CNTF), a member of the neurocytokine family, are 
      known to have synergistic effects on motoneurons, but such synergistic effect has 
      not been studied in detail especially in the brain. In the present study, we 
      examined the synergistic effects of BDNF and CNTF on the survival of basal 
      forebrain cholinergic neurons cultured from postnatal 2-week-old (P2w) rats. 
      Although BDNF is well-known to promote the survival of basal forebrain 
      cholinergic neurons in P2w culture, CNTF had little effect on the survival of 
      choline acetyltransferase (ChAT)-positive neurons and did not increase ChAT 
      activity in the culture. However, CNTF enhanced BDNF-mediated promotion of cell 
      survival of cholinergic neurons when added concomitantly. BDNF alone induced only 
      a three-fold increase in ChAT activity in control cultures, but the concomitant 
      addition of CNTF resulted in an eight-fold increase. CNTF did not enhance 
      BDNF-mediated cell survival of total neurons from the basal forebrain, 
      hippocampus or cerebellum, suggesting that the synergistic effects of CNTF on the 
      BDNF-mediated increase of viability might be strong in basal forebrain 
      cholinergic neurons. CNTF also enhanced the neurotrophin-4/5-mediated increase of 
      ChAT activity, but not the nerve growth factor (NGF)-mediated one. Furthermore, 
      the BDNF-mediated increase was also enhanced by leukemia inhibitory factor but 
      not by interleukin-6. Similar synergistic pattern between neurotrophins and 
      cytokines were also observed in the induction of ChAT activity in embryonic basal 
      forebrain culture. These results suggest that TrkB, a functional high-affinity 
      receptor of BDNF and NT-4/5, and LIFR beta, a receptor component contained in 
      CNTF and LIF receptor complex, might be involved in the observed synergistic 
      effects.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Hashimoto, Y
AU  - Hashimoto Y
AD  - Division of Protein Biosynthesis, Institute for Protein Research, Osaka 
      University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Abiru, Y
AU  - Abiru Y
FAU - Nishio, C
AU  - Nishio C
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/*physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Ciliary Neurotrophic Factor
MH  - Drug Synergism
MH  - Male
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neuroglia/physiology
MH  - Neurons/*drug effects
MH  - Prosencephalon/cytology/*drug effects
MH  - Rats
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0165380699000474 [pii]
AID - 10.1016/s0165-3806(99)00047-4 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1999 Jun 8;115(1):25-32. doi: 
      10.1016/s0165-3806(99)00047-4.