PMID- 10367379
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 82
IP  - 3
DP  - 1999 May-Jun
TI  - Determination of flumequine in channel catfish by liquid chromatography with 
      fluorescence detection.
PG  - 614-9
AB  - Rapid methods are described for determination of flumequine (FLU) residues in 
      muscle and plasma of farm-raised channel catfish (Ictalurus punctatus). FLU 
      residues were extracted from tissues with an acidified methanol solution, and 
      extracts were cleaned up on C18 solid-phase extraction cartridges. FLU 
      concentrations were determined by liquid chromatography (LC) using a C18 
      analytical column and fluorescence detection (excitation, 325 nm; emission, 360 
      nm). Mean recoveries of FLU from fortified muscle were 87-94% at 5 levels ranging 
      from 10 to 160 ppb (5 replicates per level). FLU recoveries from fortified plasma 
      were 92-97% at 5 levels ranging from 20 to 320 ppb. Limits of detection 
      (signal-to-noise ratio, 3:1) for the method as described were 3 and 6 ppb for 
      muscle and plasma, respectively. Relative standard deviations (RSDs) for 
      recoveries were < or = 12%. Live catfish were dosed with 14C-labeled or unlabeled 
      FLU to generate incurred residues. Recoveries of 14C residues throughout 
      extraction and cleanup were 90 and 94% for muscle and plasma, respectively. RSDs 
      for incurred FLU at 2 levels in muscle and plasma ranged from 2 to 6%. The 
      identity of FLU in incurred tissues was confirmed by LC/mass spectrometry.
FAU - Plakas, S M
AU  - Plakas SM
AD  - U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, 
      AL 36528, USA.
FAU - el Said, K R
AU  - el Said KR
FAU - Bencsath, F A
AU  - Bencsath FA
FAU - Musser, S M
AU  - Musser SM
FAU - Walker, C C
AU  - Walker CC
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Quinolizines)
RN  - UVG8VSP2SJ (flumequine)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*analysis/blood
MH  - Carbon Radioisotopes
MH  - Chromatography, Liquid/*methods
MH  - Drug Residues/analysis
MH  - *Fluoroquinolones
MH  - Hydrogen-Ion Concentration
MH  - *Ictaluridae
MH  - Methanol
MH  - Muscles/chemistry
MH  - Quality Control
MH  - Quinolizines/*analysis/blood
MH  - Spectrometry, Fluorescence
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1999 May-Jun;82(3):614-9.